Department of Biochemistry and Molecular Biology, School of Life Sciences, Pondicherry University, Pondicherry 605014, India.
FEMS Microbiol Lett. 2019 Apr 1;366(8). doi: 10.1093/femsle/fnz065.
We investigated the protective effect of a natural polyphenol, magnolol, on Saccharomyces cerevisiae cells under oxidative stress, and during aging. Our results showed the sensitivity of S. cerevisiae antioxidant gene deficient mutants (sod1∆, sod2∆, cta1∆, ctt1∆, gtt2∆ and tsa1∆) against hydrogen peroxide (H2O2) and menadione stress was rescued by magnolol as demonstrated in spot and colony forming unit counts. Yeast cells pretreated with magnolol showed decreased intracellular oxidation, lipid peroxidation and an increased level of reduced glutathione. Further, SOD1, CTA1 and GTT2 gene expression was examined by reverse transcription-polymerase chain reaction, and was found that magnolol significantly attenuated the upregulation of SOD1 and CTA1 genes under oxidative stress. Finally, longevity of the wild type and sod1 mutant cells were extended by magnolol, and also enhance stress resistance against oxidant stress during chronological aging.
我们研究了天然多酚厚朴酚对酿酒酵母细胞在氧化应激和衰老过程中的保护作用。结果表明，抗氧化基因缺失突变体（sod1∆、sod2∆、cta1∆、ctt1∆、gtt2∆和tsa1∆）对过氧化氢（H2O2）和甲萘醌胁迫的敏感性可以通过厚朴酚得到挽救，这一点可以从斑点和菌落形成单位计数中得到证实。经厚朴酚预处理的酵母细胞表现出细胞内氧化、脂质过氧化减少，还原型谷胱甘肽水平增加。此外，通过逆转录-聚合酶链反应检测 SOD1、CTA1 和 GTT2 基因的表达，发现厚朴酚能显著减弱氧化应激下 SOD1 和 CTA1 基因的上调。最后，厚朴酚延长了野生型和 sod1 突变细胞的寿命，并增强了其在时序老化过程中对氧化应激的抵抗能力。
