Lu Pan-Jian, Wang Gang, Cai Xiao-Dong, Zhang Ping, Wang Hong-Kui
Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China.
Neural Regen Res. 2020 Nov;15(11):2116-2122. doi: 10.4103/1673-5374.282263.
Previous research revealed the positive activity of matrix metalloproteinase 7 (MMP7) on migration and myelin regeneration of Schwann cells (SCs). However, understanding of the molecular changes and biological activities induced by increased amounts of MMP7 in SCs remains limited. To better understand the underlying molecular events, primary SCs were isolated from the sciatic nerve stump of newborn rats and cultured with 10 nM human MMP7 for 24 hours. The results of genetic testing were analyzed at a relatively relaxed threshold value (fold change ≥ 1.5 and P-value < 0.05). Upon MMP7 exposure, 149 genes were found to be upregulated in SCs, whereas 133 genes were downregulated. Gene Ontology analysis suggested that many differentially expressed molecules were related to cellular processes, single-organism processes, and metabolic processes. Kyoto Enrichment of Genes and Genomes pathway analysis further indicated the critical involvement of cell signaling and metabolism in MMP7-induced molecular regulation of SCs. Results of Ingenuity Pathway Analysis (IPA) also revealed that MMP7 regulates biological processes, molecular functions, cellular components, diseases and functions, biosynthesis, material metabolism, cell movement, and axon guidance. The outcomes of further analysis will deepen our comprehension of MMP7-induced biological changes in SCs. This study was approved by the Laboratory Animal Ethics Committee of Nantong University, China (approval No. 20190225-004) on February 27, 2019.
先前的研究揭示了基质金属蛋白酶7（MMP7）对雪旺细胞（SCs）迁移和髓鞘再生的积极作用。然而，对于SCs中MMP7含量增加所诱导的分子变化和生物学活性的了解仍然有限。为了更好地理解潜在的分子事件，从新生大鼠的坐骨神经残端分离出原代SCs，并用10 nM人MMP7培养24小时。在相对宽松的阈值（倍数变化≥1.5且P值<0.05）下分析基因检测结果。暴露于MMP7后，发现SCs中有149个基因上调，而133个基因下调。基因本体分析表明，许多差异表达分子与细胞过程、单细胞过程和代谢过程有关。京都基因与基因组百科全书通路分析进一步表明，细胞信号传导和代谢在MMP7诱导的SCs分子调控中起关键作用。 Ingenuity通路分析（IPA）结果还显示，MMP7调节生物学过程、分子功能、细胞成分、疾病和功能、生物合成、物质代谢、细胞运动和轴突导向。进一步分析的结果将加深我们对MMP7诱导的SCs生物学变化的理解。本研究于2019年2月27日获得中国南通大学实验动物伦理委员会批准（批准号：20190225-004）。
