Brutel de la Rivière A, Verhoef-Karssen P R, von dem Borne A E, Engelfriet C P
Scand J Immunol. 1977;6(4):349-56. doi: 10.1111/j.1365-3083.1977.tb00404.x.
In view of the importance of the species of animals used for the preparation of specific heteroantisera against human blood cells, comparative immunization experiments were carried out in goats, rabbit, chickens, guinea pigs, and rats, using lysates of leukocytes, granulocytes, lymphocytes, monocytes, and thrombocytes. The antisera obtained were tested by the indirect immunofluorescence technique. The strongest antisera were obtained in goats, but these antisera required extensive absorptions to make them specific for the immunizing cell, whereas rabbit antisera needed only few absorptions. Antisera from both of these animals could be rendered specific for lymphocytes, granulocytes and platelets. In the guinea pig only immunization with monocytes and granulocytes resulted in useful antisera, which were all specific for granulocytes after appropriate absorptions. Antisera obtained from rats could be rendered specific for granulocytes and lymphocytes by absorption. Antibodies against platelets could not be detected in any of the rat antisera. Chickens, however, produced strong anti-platelet antibodies. A specific anti-granulocyte antiserum could also be obtained in these animals. Specific anti-monocyte antisera could not be prepared from any of the animal sera. Immunizations with leukocyte lysate resulted in granulocyte-specific antisera in most animals but not in chickens. All antisera produced in chickens were specific for platelets. It can be concluded from these results that animals of different species may react very differenly to the antigens of some human blood cells.
鉴于用于制备针对人类血细胞的特异性异种抗血清所使用动物种类的重要性，我们使用白细胞、粒细胞、淋巴细胞、单核细胞和血小板的裂解物，在山羊、兔子、鸡、豚鼠和大鼠身上进行了比较免疫实验。通过间接免疫荧光技术对获得的抗血清进行检测。在山羊身上获得的抗血清最强，但这些抗血清需要大量吸收处理才能使其对免疫细胞具有特异性，而兔抗血清只需要少量吸收处理。这两种动物的抗血清都可以针对淋巴细胞、粒细胞和血小板变得具有特异性。在豚鼠中，只有用单核细胞和粒细胞进行免疫才产生了有用的抗血清，经过适当吸收处理后，这些抗血清都对粒细胞具有特异性。从大鼠获得的抗血清通过吸收处理可以针对粒细胞和淋巴细胞变得具有特异性。在任何大鼠抗血清中都未检测到针对血小板的抗体。然而，鸡产生了强烈的抗血小板抗体。在这些动物中也可以获得特异性的抗粒细胞抗血清。无法从任何动物血清中制备出特异性的抗单核细胞抗血清。用白细胞裂解物进行免疫在大多数动物中产生了针对粒细胞的特异性抗血清，但在鸡中没有。鸡产生的所有抗血清都对血小板具有特异性。从这些结果可以得出结论，不同物种的动物对某些人类血细胞抗原的反应可能非常不同。
