Brutel de la Rivière A, Verhoef-Karssen P R, Bosma A, Kr vd Borne A E, Engelfriet P
Scand J Immunol. 1976;5(9):1065-74. doi: 10.1111/j.1365-3083.1976.tb03058.x.
The preparation of antisera against various cells of the human peripheral blood applicable in the indirect immunofluorescence technique (IIFT) is described. Such antisera will be a great interest for the study of cell-specific membrane antigens, for example during haematopoiesis. Purified erythrocytes, lymphocytes, neutrophils, monocytes, and thrombocytes from healthy donors were injected into rabbits. The antisera thus produced were not spontaneously specific. Only by extensive absorption of the crude antisera with purified cells from healthy donors was it possible to obtain antisera that were specific for erythrocytes, lymphocytes, and thrombocytes. By injection of small doses of leukocyte lysate and by absorption of the resulting antiserum with mononclear cells a specific antineutrophil serum was produced. So far it has not been possible to prepare a specific anti-monocyte antiserum. The specific antisera were applicable in the IIFT on paraformaldehyde-fixed cells in suspension and on cells on slides.
本文描述了适用于间接免疫荧光技术（IIFT）的抗人外周血各种细胞抗血清的制备方法。此类抗血清对于研究细胞特异性膜抗原具有重要意义，例如在造血过程中。将来自健康供体的纯化红细胞、淋巴细胞、中性粒细胞、单核细胞和血小板注射到兔子体内。由此产生的抗血清并非自发具有特异性。只有通过用来自健康供体的纯化细胞对粗抗血清进行广泛吸收，才有可能获得对红细胞、淋巴细胞和血小板具有特异性的抗血清。通过注射小剂量白细胞裂解物并使用单核细胞对所得抗血清进行吸收，制备出了特异性抗中性粒细胞血清。到目前为止，尚未成功制备出特异性抗单核细胞抗血清。这些特异性抗血清可用于悬浮状态下经多聚甲醛固定的细胞以及载玻片上细胞的间接免疫荧光技术检测。
