DeVries Aaron E, Kowalski Kurt P, Bickford Wesley A
USGS Great Lakes Science Center, Ann Arbor, MI 48105, USA.
Microorganisms. 2020 May 8;8(5):690. doi: 10.3390/microorganisms8050690.
subsp is a cosmopolitan wetland grass that is invasive in many regions of the world, including North America, where it co-occurs with the closely related subsp. Because the difference in invasive behavior is unlikely to be related to physiological differences, we hypothesize that interactions with unique members of their microbiomes may significantly affect the behavior of each subspecies. Therefore, we systematically inoculated both plant lineages with a diverse array of 162 fungal and bacterial isolates to determine which could (1) differentiate between hosts, (2) infect leaves at various stages of development, or (3) obtain plant-based carbon saprophytically. We found that many of the microbes isolated from leaves behave as saprophytes. Only 1% (two taxa) were determined to be strong pathogens, 12% (20 taxa) were weakly pathogenic, and the remaining 87% were nonpathogenic. None of the isolates clearly discriminated between host plant lineages, and the cuticle was shown to be a strong nonspecific barrier to infection. These results largely agree with the broad body of literature on leaf-associated phyllosphere microbes in .
亚种是一种遍布全球的湿地草，在世界许多地区都具有入侵性，包括北美，在那里它与近缘亚种共生。由于入侵行为的差异不太可能与生理差异有关，我们推测与它们独特的微生物群落成员的相互作用可能会显著影响每个亚种的行为。因此，我们用162种多样的真菌和细菌分离株系统地接种了这两个植物谱系，以确定哪些分离株能够（1）区分宿主，（2）在不同发育阶段感染叶片，或（3）以腐生方式获取植物源碳。我们发现，从叶片中分离出的许多微生物表现为腐生菌。只有1%（两个分类单元）被确定为强病原菌，12%（20个分类单元）为弱病原菌，其余87%为非病原菌。没有一个分离株能清楚地区分宿主植物谱系，并且角质层被证明是感染的一个强大的非特异性屏障。这些结果在很大程度上与关于叶际微生物的大量文献一致。
