Piras Ilaria M, Dei Giudici Silvia, Fadda Manlio, Anfossi Antonio G, Oggiano Annalisa, Pittau Marco, Chessa Bernardo
Department of Veterinary Medicine, University of Sassari, 07100 Sassari, Italy.
Istituto Zooprofilattico Sperimentale della Sardegna, 07100 Sassari, Italy.
Pathogens. 2020 May 9;9(5):360. doi: 10.3390/pathogens9050360.
Border Disease (BD) is a worldwide distributed pathology accountable for significant losses in the sheep and goat farming industry. The etiological agent is a within the family called (BDV). Despite the Sardinian ovine population being by far larger than any other Italian region, the prevalence and distribution of BD on the island are unknown. Here, we aim to determine the distribution of BDV in sheep flocks and to genetically characterize the circulating strains in Sardinia. The geographical distribution, antibody positivity, and viral genome presence have been analysed for 1286 sheep flocks distributed all over the island from bulk tank milk sampled between May 2014 and 2015. Of the flocks tested, 11.28% (95% CI 9.66-13.12) resulted positive for the presence of anti-pestivirus antibodies with an uneven distribution between Sardinian provinces. In addition, using RT-PCR, nine BDV genomes were amplified from milk pellets of the seropositive samples. Phylogenetic analysis revealed that all the viruses amplified clustered in the same group classified as BDV-7. This represents the first study on the distribution of pestivirus infection and genetic characterization of BDV strains circulating in the Sardinian sheep population. Future studies are needed to clarify the origin, the evolution, and the epidemiology of BDV-7 in Sardinia.
边界病（BD）是一种在全球范围内分布的病理状况，给绵羊和山羊养殖业造成了重大损失。其病原体是黄病毒科中的一种病毒，称为边界病病毒（BDV）。尽管撒丁岛的绵羊数量远远超过意大利的任何其他地区，但该岛BD的流行情况和分布尚不清楚。在此，我们旨在确定BDV在绵羊群中的分布，并对撒丁岛流行毒株进行基因特征分析。我们对2014年5月至2015年期间采集的全岛1286个绵羊群的散装罐奶进行了地理分布、抗体阳性率和病毒基因组存在情况分析。在所检测的羊群中，11.28%（95%置信区间9.66 - 13.12）的抗瘟病毒抗体检测呈阳性，在撒丁岛各省之间分布不均。此外，通过逆转录聚合酶链反应（RT-PCR），从血清阳性样本的奶沉淀物中扩增出9个BDV基因组。系统发育分析表明，所有扩增出的病毒都聚集在同一组，归类为BDV - 7。这是关于撒丁岛绵羊群体中瘟病毒感染分布及BDV毒株基因特征的首次研究。未来需要开展研究以阐明撒丁岛BDV - 7的起源、进化和流行病学情况。
