Rosamilia A, Grattarola C, Caruso C, Peletto S, Gobbi E, Tarello V, Caroggio P, Dondo A, Masoero L, Acutis P L
Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Torino, Italy.
Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Torino, Italy.
Vet J. 2014 Mar;199(3):446-50. doi: 10.1016/j.tvjl.2013.12.006. Epub 2013 Dec 11.
In January 2011, cases of abortion, stillbirth and weak live kids were reported in two goat herds in northern Italy. Samples from 18 kids found dead, 12 fetuses, and two stillborn kids were analyzed for pestivirus antigen using an ELISA kit and a border disease virus (BDV)-specific RT-PCR. Positive results were obtained in six kids and one fetus. Phylogenetic analysis based on 225 bp of the 5'UTR fragment of the BDV genome from positive samples showed that the goats were infected with BDV genotype 3. Serum and blood samples collected from all animals in both herds were analyzed using competitive ELISA to detect p80 antibodies and RT-PCR to detect viraemia. Pestivirus antibodies were detected in 61/67 goats in herd A and in 38/169 in herd B. A persistently infected (PI) goat was found in herd A. The PI animal was submitted to the laboratory for BDV diagnosis with Ag-ELISA, viral isolation, and nested RT-PCR on tissue samples from the spleen, kidney, brain, liver, lung, ileocaecal valve, mesenteric lymph nodes, and skin. All of the tests were positive for BDV in each of the tissues analyzed. The BDV sequence of the PI was identical to BDV sequences found in other positive animals. This is the first description of a BDV PI goat and the first evidence of BDV genotype 3 circulation in Italy. The study raises questions about the real impact this virus has on breeding goats.
2011年1月，意大利北部两个山羊群中报告出现流产、死产和弱仔病例。使用酶联免疫吸附测定试剂盒和边境病病毒（BDV）特异性逆转录聚合酶链反应（RT-PCR）对18只死亡羔羊、12个胎儿和2只死产羔羊的样本进行了瘟病毒抗原分析。6只羔羊和1个胎儿检测呈阳性。基于阳性样本中BDV基因组5'非翻译区（UTR）片段225 bp进行的系统发育分析表明，山羊感染了BDV 3型。使用竞争酶联免疫吸附测定法分析了两个羊群中所有动物采集的血清和血液样本，以检测p80抗体，并使用RT-PCR检测病毒血症。在A群的67只山羊中有61只检测到瘟病毒抗体，在B群的169只山羊中有38只检测到瘟病毒抗体。在A群中发现了一只持续感染（PI）的山羊。将这只PI动物送交实验室，通过抗原酶联免疫吸附测定、病毒分离以及对来自脾脏、肾脏、大脑、肝脏、肺、回盲瓣、肠系膜淋巴结和皮肤的组织样本进行巢式RT-PCR来进行BDV诊断。在所分析的每个组织中，所有检测对BDV均呈阳性。这只PI山羊的BDV序列与在其他阳性动物中发现的BDV序列相同。这是对BDV PI山羊的首次描述，也是BDV 3型在意大利传播的首个证据。该研究引发了关于这种病毒对种用山羊实际影响的疑问。
