Wang Si, Hu Ming, Chen Huilin, Li Chuhao, Xue Yang, Song Xinyue, Qi Yuqing, Liu Fan, Zhou Xiaofan, Zhang Lian-Hui, Zhou Jianuan
National Key Laboratory of Green Pesticide, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Center, South China Agricultural University, Guangzhou, China.
Front Plant Sci. 2023 Jun 30;14:1193297. doi: 10.3389/fpls.2023.1193297. eCollection 2023.
Bacterial wilt caused by ranks the second top important bacterial plant disease worldwide. It is also the most important bacterial disease threatening the healthy development of protection forest. 3-hydroxypalmitic acid methyl ester (3-OH PAME) functions as an important quorum sensing (QS) signal regulating the expression of virulence genes in , and has been regarded as an ideal target for disease prevention and control. To screen native microorganisms capable of degrading 3-OH PAME, samples of branches and forest soil were collected and cultured in the medium containing 3-OH PAME as the sole carbon source. Bacteria with over 85% degradation rates of 3-OH PAME after 7-day incubation were further separated and purified. As a result, strain Q1-7 isolated from forest soil and strain Q4-3 isolated from branches were obtained and identified as novel species sp. nov. and , respectively, according to whole genome sequencing results. The degradation efficiencies of 3-OH PAME of strains Q1-7 and Q4-3 were 95.80% and 100.00% at 48 h, respectively. Both strains showed high esterase activities and inhibited exopolysaccharide (EPS) and cellulase production. Application of strains Q1-7 and Q4-3 effectively protects , peanut and tomato plants from infection by . Findings in this study provide potential resources for the prevention and control of bacterial wilt caused by , as well as valuable materials for the identification of downstream quenching genes and the research and development of quenching enzymes for disease control.
由[病原菌名称未给出]引起的青枯病是全球第二大重要的细菌性植物病害。它也是威胁防护林健康发展的最重要的细菌性病害。3-羟基棕榈酸甲酯（3-OH PAME）作为一种重要的群体感应（QS）信号，调节[病原菌名称未给出]中致病基因的表达，被视为疾病预防和控制的理想靶点。为了筛选能够降解3-OH PAME的天然微生物，采集了[植物名称未给出]枝条和森林土壤样本，并在以3-OH PAME为唯一碳源的培养基中培养。对培养7天后3-OH PAME降解率超过85%的细菌进行进一步分离和纯化。结果，从森林土壤中分离出的菌株Q1-7和从[植物名称未给出]枝条中分离出的菌株Q4-3，根据全基因组测序结果，分别被鉴定为新物种[新物种名称1] sp. nov. 和[新物种名称2]。菌株Q1-7和Q4-3在48小时时对3-OH PAME的降解效率分别为95.80%和100.00%。两种菌株均表现出高酯酶活性，并抑制[病原菌名称未给出]胞外多糖（EPS）和纤维素酶的产生。菌株Q1-7和Q4-3的应用有效地保护了[植物名称未给出]、花生和番茄植株免受[病原菌名称未给出]的感染。本研究结果为[病原菌名称未给出]引起的青枯病的预防和控制提供了潜在资源，也为下游淬灭基因的鉴定以及用于疾病控制的淬灭酶的研发提供了有价值的材料。
