Tan Xiaojuan, Ju Guangyu, Feng Dandan, Chen Liuji, Fan Xiaodie, Zhu Cancan, Li Minghui, Du Dongsheng
College of Life Sciences, Anhui Normal University, Wuhu, 241000, Anhui, China.
Int Microbiol. 2025 May 30. doi: 10.1007/s10123-025-00676-1.
Pyocyanin is a crucial virulence factor uniquely secreted by Pseudomonas aeruginosa. Hence, the rapid and selective detection of pyocyanin can reveal essential information about the pathogenesis of diseases caused by clinical isolates. Herein, we found that P. aeruginosa PAO1 exhibited remarkable antagonistic activity against Dickeya zeae WH1. Subsequently, its active compounds were purified by preparative high-performance liquid chromatography and HPLC, and anti-WH1 activity was determined by performing the minimum inhibitory concentration (MIC) assay. Only one active compound, pyocyanin, was obtained. Importantly, the linear quantification of pyocyanin to the biomass (OD600) of WH1 showed an R value of 0.9908 ranging from 0.195 to 6.25 µg/mL, and pyocyanin concentration was accurately determined using this curve when P. aeruginosa was cultured in high-, medium-, and low-pyocyanin-producing media. Therefore, using D. zeae WH1 as a sensor for detecting pyocyanin and high-pyocyanin-producing isolates by high-throughput screening is a cost-effective and simple method.
绿脓菌素是铜绿假单胞菌独特分泌的一种关键毒力因子。因此，快速、选择性地检测绿脓菌素可以揭示有关临床分离株所引起疾病发病机制的重要信息。在此，我们发现铜绿假单胞菌PAO1对玉米细菌性枯萎病菌WH1表现出显著的拮抗活性。随后，通过制备型高效液相色谱和高效液相色谱对其活性化合物进行了纯化，并通过进行最低抑菌浓度（MIC）测定来确定抗WH1活性。仅获得了一种活性化合物，即绿脓菌素。重要的是，绿脓菌素与WH1生物量（OD600）的线性定量分析显示，在0.195至6.25 µg/mL范围内，R值为0.9908，并且当铜绿假单胞菌在高、中、低绿脓菌素产生培养基中培养时，使用该曲线可准确测定绿脓菌素浓度。因此，利用玉米细菌性枯萎病菌WH1作为传感器通过高通量筛选检测绿脓菌素和高产绿脓菌素分离株是一种经济高效且简便的方法。
