Food Microbiology Research Laboratories, R&D Division, Meiji Co., Ltd, Tokyo, Japan.
Faculty of Pharma Sciences, Teikyo University, Tokyo, Japan.
Nucleosides Nucleotides Nucleic Acids. 2020;39(10-12):1440-1448. doi: 10.1080/15257770.2020.1733604. Epub 2020 May 13.
Although most lactic acid bacteria do not directly incorporate purine nucleotides, the strain PA-3 was found to incorporate purine mononucleotides. To determine whether the direct uptake of purine mononucleotides is dependent on the species or strain of lactic acid bacteria, incorporation of purine mononucleotides was assessed in , sbsp. and other species of lactic acid bacteria. Each bacterial strain was incubated with P-AMP or C-adenosine and the incorporation of each purine was evaluated by measuring their radioactivity. All investigated strains of incorporated P-AMP, whereas strains of and most strains of did not. Incorporation of P-AMP into strains of was dependent on the strain or species of that genus of bacteria. All investigated strains, except for one strain of , incorporated C-adenosine, with , and generally displaying greater incorporation of C-adenosine than . Although most lactic acid bacteria such as and do not incorporate purine mononucleotides, some species such as directly incorporate purine mononucleotides. These findings indicate that the preferential incorporation of purine mononucleotides or nucleosides by lactic acid bacteria is dependent on the species or strain.
虽然大多数乳酸菌并不直接摄取嘌呤核苷酸，但我们发现菌株 PA-3 可以摄取嘌呤单核苷酸。为了确定直接摄取嘌呤单核苷酸是否依赖于乳酸菌的种类或菌株，我们评估了不同乳酸菌亚种、和其他乳酸菌属的种对嘌呤单核苷酸的摄取情况。将每种细菌菌株与 P-AMP 或 C-腺苷孵育，并通过测量它们的放射性来评估每种嘌呤的摄取情况。所有被调查的菌株都摄取了 P-AMP，而 和大多数 菌株则没有。摄取 P-AMP 取决于该属的菌株或种类。除了一种 菌株外，所有被调查的菌株都摄取了 C-腺苷，其中 、 和 通常比 摄取更多的 C-腺苷。尽管大多数乳酸菌如 和 不摄取嘌呤单核苷酸，但有些物种如 可以直接摄取嘌呤单核苷酸。这些发现表明，乳酸菌对嘌呤单核苷酸或核苷的优先摄取取决于其种类或菌株。
