Kano H, Yamada N, Saito C, Murayama-Chiba Y, Asami Y, Ito H
a Food Microbiology Research Laboratories, R&D Division , Meiji Co., Ltd. Tokyo , Japan.
Nucleosides Nucleotides Nucleic Acids. 2018;37(6):353-360. doi: 10.1080/15257770.2018.1469760. Epub 2018 May 29.
Lactobacillus gasseri PA-3 (PA-3) is a bacterial strain with a strong ability to degrade purine nucleosides. We previously showed that PA-3 incorporates purines in vitro and that oral administration of PA-3 and purines to rats attenuated their absorption of purines. It remains unclear whether these effects of PA-3 depend on bacterial strains. This study therefore compared the abilities of PA-3 and another bacterial strain of L. gasseri, OLL2996, which has shown decreased ability to degrade purine nucleosides in vitro, to incorporate purine nucleosides and to inhibit the absorption of purines fed to rats. Each bacterial strain was incubated in the presence of C-adenosine or C-inosine and the incorporation of each purine was evaluated by measuring their radioactivity. In vivo, rats were fed C-labeled purines along with PA-3 or OLL2996 and the absorption of these C-labeled purines was evaluated by analyzing radioactivity of blood samples. PA-3 incorporated about twice as much C-adenosine and C-inosine as OLL2996. The elevation of radioactivity levels in blood was 10-20% lower in rats treated with PA-3 than in control rats, after feeding with both C-adenosine and C-inosine as purines. In contrast, treatment with OLL2996 did not have statistically significant effects on radioactivity compared with the control group. These results indicate that the magnitude of bacterial inhibition of purine absorption is dependent on bacterial strain, correlating at least partly with the ability to incorporate and degrade purines.
加氏乳杆菌PA - 3（PA - 3）是一种具有强大嘌呤核苷降解能力的菌株。我们之前表明，PA - 3在体外能摄取嘌呤，并且给大鼠口服PA - 3和嘌呤可减弱它们对嘌呤的吸收。目前尚不清楚PA - 3的这些作用是否取决于菌株。因此，本研究比较了PA - 3与加氏乳杆菌的另一个菌株OLL2996摄取嘌呤核苷以及抑制大鼠摄入嘌呤吸收的能力，OLL2996在体外已显示出嘌呤核苷降解能力下降。将每种菌株在C - 腺苷或C - 肌苷存在的情况下进行培养，并通过测量其放射性来评估每种嘌呤的摄取情况。在体内，给大鼠喂食C标记的嘌呤以及PA - 3或OLL2996，通过分析血样的放射性来评估这些C标记嘌呤的吸收情况。PA - 3摄取的C - 腺苷和C - 肌苷量约为OLL2996的两倍。在用C - 腺苷和C - 肌苷作为嘌呤喂食后，用PA - 3处理的大鼠血液中放射性水平的升高比对照大鼠低10 - 20%。相比之下，与对照组相比，用OLL2996处理对放射性没有统计学上的显著影响。这些结果表明，细菌对嘌呤吸收的抑制程度取决于菌株，至少部分与摄取和降解嘌呤的能力相关。
