College of Veterinary Medicine, Northeast Agricultural University, Heilongjiang, China.
Animal Health and Diagnostic Center of CP Group, Heilongjiang, China.
Viral Immunol. 2020 Oct;33(8):550-554. doi: 10.1089/vim.2020.0025. Epub 2020 May 12.
Pseudorabies (PR) is a highly contagious disease affecting a wide range of animals, which annually causes great economic losses in China. In this study, a total number of 18,815 serum samples and 1,589 tissue samples were collected from 311 intensive pig farms (≥350 sows) located in eight cities in Heilongjiang province, and tested by ELISA and PCR. Overall, the serum positive rates of gE and gB protein were 16.3% and 84.5%, respectively. The PCR-positive rate of PR virus (PRV) in tissue samples was 17.8%. The coinfection rates of PRV with porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus type 2 (PCV2), and classical swine fever virus (CSFV) were also measured. The rate of PRV coinfected with PRRSV was 36.0% followed by 12.9% with PCV2 and 1.8% with CSFV, respectively. These results clearly demonstrate PRV prevalence and its coinfection rate in Heilongjiang province, indicating high PR endemic in pig farms in this region. This study provides data for further epidemiological investigations and a reference for developing PRV control strategies in this region and in China.
伪狂犬病（PR）是一种高度传染性疾病，影响范围广泛的动物，每年在中国造成巨大的经济损失。在这项研究中，从黑龙江省八个城市的 311 个集约化养猪场（≥350 头母猪）共采集了 18815 份血清样本和 1589 份组织样本，通过 ELISA 和 PCR 进行检测。总体而言，gE 和 gB 蛋白的血清阳性率分别为 16.3%和 84.5%。组织样本中 PR 病毒（PRV）的 PCR 阳性率为 17.8%。还测量了 PRV 与猪繁殖与呼吸综合征病毒（PRRSV）、猪圆环病毒 2 型（PCV2）和经典猪瘟病毒（CSFV）的混合感染率。PRV 与 PRRSV 的混合感染率为 36.0%，其次是与 PCV2 的混合感染率为 12.9%，与 CSFV 的混合感染率为 1.8%。这些结果清楚地表明了黑龙江省 PRV 的流行情况及其混合感染率，表明该地区猪场的 PR 呈地方性流行。本研究为进一步的流行病学调查提供了数据，并为该地区和中国制定 PRV 控制策略提供了参考。
