State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China.
Vet Med Sci. 2021 Jan;7(1):175-183. doi: 10.1002/vms3.289. Epub 2020 Jun 24.
Porcine respiratory disease complex (PRDC), a respiratory disease caused by a variety of factors, is one of the most common problems in the intensive pig farms. To investigate the mixed infection incidence of wild-type pseudorabies virus (WT PRV) and respiratory bacteria, a total of 1,293 clinical samples were collected from pigs with typical respiratory signs from 14 different provinces of China from September 2016 to February 2018. The WT PRV was detected by ELISA targeting gE antibody while the bacteria were detected by bacterial isolation and serotyping by PCR. The results revealed that the detection rate of A. pleuropneumoniae and B. bronchiseptica infection associated with WT PRV infection were 6.30% and 15.99%, respectively, which were significantly higher than those without WT PRV infection (3.41% and 4.41%) at the farm level (p < .05). There were no significant differences in the detection rate of H. parasuis, S. suis or P. multocida between WT PRV positive and negative farms (p > .05). However, the detection rate of attenuated H. parasuis and S. suis strains were 68.19% and 64.75%, respectively, in WT PRV infected farms, which were significantly higher than those (41.56% and 52.25%) in WT PRV free farms (p < .05). The prevalent serotypes of H. parasuis-5/12 and S. suis-2 were also investigated by multiplex PCR. These results indicated that the presence of WT PRV increased the chance of bacterial infection and the number of pathogenic strains in the respiratory system of pigs. Therefore, the eradication of pseudorabies is an effective approach to prevent and control the bacterial respiratory diseases in the intensive pig farms in China.
猪呼吸道疾病综合征（PRDC）是一种由多种因素引起的呼吸道疾病，是集约化猪场最常见的问题之一。为了调查野毒株伪狂犬病病毒（WT PRV）与呼吸道细菌的混合感染发生率，本研究于 2016 年 9 月至 2018 年 2 月，从中国 14 个不同省份的具有典型呼吸道症状的猪群中采集了 1293 份临床样本。采用 ELISA 检测 gE 抗体的方法检测 WT PRV，通过细菌分离和 PCR 血清型鉴定的方法检测细菌。结果显示，与 WT PRV 感染相关的胸膜肺炎放线杆菌和支气管败血波氏杆菌的感染检出率分别为 6.30%和 15.99%，显著高于 WT PRV 阴性场的检出率（3.41%和 4.41%）（p<0.05）。在 WT PRV 阳性场和阴性场之间，副猪嗜血杆菌、猪链球菌或多杀性巴氏杆菌的检出率无显著差异（p>0.05）。然而，在 WT PRV 感染场，减毒副猪嗜血杆菌和猪链球菌的检出率分别为 68.19%和 64.75%，显著高于 WT PRV 阴性场的检出率（41.56%和 52.25%）（p<0.05）。通过多重 PCR 还检测了副猪嗜血杆菌-5/12 型和猪链球菌-2 型的流行血清型。这些结果表明，WT PRV 的存在增加了猪呼吸道系统细菌感染和致病性菌株的机会。因此，根除伪狂犬病是预防和控制中国集约化猪场细菌性呼吸道疾病的有效方法。
