Yamkamon Vichanan, Htoo Khin Phyu Pyar, Yainoy Sakda, Suksrichavalit Thummaruk, Tangchaikeeree Tienrat, Eiamphungporn Warawan
Department of Clinical Microscopy, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand.
Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand.
EXCLI J. 2020 Apr 15;19:501-513. doi: 10.17179/excli2020-1036. eCollection 2020.
is one of the most prostate cancer-specific genes described to date. Of note, expression is detectable at high level in the urine of prostate cancer (PCa) patients. Accordingly, is an ideal biomarker for PCa diagnosis. Several techniques for the measurement of this biomarker in urine have been developed but there are still some drawbacks. In this study, magnetic nanoparticle-based PCR coupled with streptavidin-horseradish peroxidase and a substrate for colorimetric detection was established as a potential assay for urinary detection. The method provided a high specificity for gene in LNCaP prostate cancer cell line. Additionally, this technique could detect at femtogram level which was approximately 1,000-fold more sensitive than the conventional RT-PCR followed by agarose gel electrophoresis. The effectiveness of the method was assessed by detection in clinical specimens. The relative expression of PCa patients determined by this assay was significantly greater than that of benign prostatic hyperplasia (BPH) patients and healthy controls. The results of our test were comparable with the results of qRT-PCR. The proposed method is promising to distinguish between cancerous and non-cancerous groups. Altogether, this simple assay is practicable and useful for prostate cancer diagnosis.
是迄今为止所描述的最具前列腺癌特异性的基因之一。值得注意的是，在前列腺癌（PCa）患者的尿液中可检测到高水平的表达。因此，是PCa诊断的理想生物标志物。已经开发了几种用于测量尿液中这种生物标志物的技术，但仍存在一些缺点。在本研究中，基于磁性纳米颗粒的PCR与链霉亲和素 - 辣根过氧化物酶以及用于比色检测的底物相结合，被确立为一种潜在的尿液检测方法。该方法对LNCaP前列腺癌细胞系中的基因具有高特异性。此外，该技术能够在飞克水平检测，其灵敏度比传统的RT-PCR随后进行琼脂糖凝胶电泳高约1000倍。通过在临床标本中进行检测来评估该方法的有效性。通过该检测方法测定的PCa患者的相对表达明显高于良性前列腺增生（BPH）患者和健康对照。我们测试的结果与qRT-PCR的结果相当。所提出的方法有望区分癌性和非癌性组。总之这种简单的检测方法对前列腺癌诊断是可行且有用的。
