Kim Miyeon, Bae Yun Kyung, Um Soyoun, Kwon Ji Hye, Kim Gee-Hye, Choi Soo Jin, Oh Wonil, Jin Hye Jin
Biomedical Research Institute, MEDIPOST Co., Ltd., Seongnam 13494, Republic of Korea.
Stem Cells Int. 2020 Apr 28;2020:5924983. doi: 10.1155/2020/5924983. eCollection 2020.
Mesenchymal stem cells (MSCs) represent a promising means to promote tissue regeneration. However, the heterogeneity of MSCs impedes their use for regenerative medicine. Further investigation of this phenotype is required to develop cell therapies with improved clinical efficacy. Here, a small-sized population of human umbilical cord blood-derived MSCs (UCB-MSCs) was isolated using a filter and centrifuge system to analyze its stem cell characteristics. Consequently, this population showed higher cell growth and lower senescence. Additionally, it exhibited diverse stem cell properties including differentiation, stemness, and adhesion, as compared to those of the population before isolation. Using cell surface protein array or sorting analysis, both EGFR and CD49f were identified as markers associated with the small-sized population. Accordingly, suppression of these surface proteins abolished the superior characteristics of this population. Moreover, compared to that with large or nonisolated populations, the small-sized population showed greater therapeutic efficacy by promoting the engraftment potential of infused cells and reducing lung damage in an emphysema mouse model. Therefore, the isolation of this small-sized population of UCB-MSCs could be a simple and effective way to enhance the efficacy of cell therapy.
间充质干细胞（MSCs）是促进组织再生的一种有前景的手段。然而，MSCs的异质性阻碍了它们在再生医学中的应用。需要对这种表型进行进一步研究，以开发具有更高临床疗效的细胞疗法。在此，使用过滤和离心系统分离出一小部分人脐带血来源的MSCs（UCB-MSCs），以分析其干细胞特性。结果，这部分细胞表现出更高的细胞生长率和更低的衰老程度。此外，与分离前的细胞群体相比，它表现出多种干细胞特性，包括分化、干性和黏附。通过细胞表面蛋白阵列或分选分析，表皮生长因子受体（EGFR）和整合素α6（CD49f）均被鉴定为与这一小部分细胞相关的标志物。因此，抑制这些表面蛋白会消除这部分细胞的优越特性。此外，与大细胞群体或未分离的细胞群体相比，这一小部分细胞通过提高注入细胞的植入潜力和减少肺气肿小鼠模型中的肺损伤，显示出更大的治疗效果。因此，分离这一小部分UCB-MSCs可能是提高细胞治疗疗效的一种简单有效的方法。
