Department of Medicine, The Pennsylvania State University College of Medicine, Hershey, PA, USA.
Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, PA, USA.
Methods Mol Biol. 2021;2346:63-71. doi: 10.1007/7651_2020_292.
Methods for the mechanistic investigations on renal fibrosis have long been concentrated on individual type of cells, such as fibroblasts and epithelial cells. However, in recent years, growing numbers of studies have been shifting toward the role of the intercellular interactions, such as communication between tubular epithelial cells and fibroblasts. Various co-culture models have been utilized in the studies of cell-cell communication and interaction. In this chapter, we describe an innovative co-culture model employing the porous membranes for spatially partitioning the cells while allowing direct crosstalk between fibroblasts and epithelial cells in an effort of mimicking in vivo environment.
方法的机械调查肾纤维化长期以来一直集中在个别类型的细胞，如成纤维细胞和上皮细胞。然而，近年来，越来越多的研究已经转向细胞间相互作用的作用，如肾小管上皮细胞和成纤维细胞之间的通信。各种共培养模型已被用于细胞-细胞通讯和相互作用的研究。在本章中，我们描述了一种创新的共培养模型，该模型采用多孔膜将细胞分隔开，同时允许成纤维细胞和上皮细胞之间直接串扰，以模拟体内环境。
