基于转录组和代谢组学的结合，从酶基因到代谢物探讨内生真菌对高山红景天的调控作用。

Regulation by fungal endophyte of Rhodiola crenulata from enzyme genes to metabolites based on combination of transcriptome and metabolome.

机构信息

Institute of Applied Chemistry, Shanxi University, Taiyuan, People's Republic of China.

Department of Food Science and Technology, College of Food, Agricultural and Environmental Sciences, The Ohio State University, Columbus, OH, USA.

出版信息

J Sci Food Agric. 2020 Sep;100(12):4483-4494. doi: 10.1002/jsfa.10489. Epub 2020 Jun 5.

DOI:10.1002/jsfa.10489

PMID:32399987

Abstract

BACKGROUND

The contents of some its crucial metabolites tend to decrease when Rhodiola crenulata is cultured at low altitude. Interestingly, it was found that an endophyte, Phialocephala fortinii, could alleviate this problem.

RESULTS

There were 16 151 differential genes including 14 706 up-regulated and 1445 down-regulated unigenes with significant differences (P < 0.05), and a total of 1432 metabolites exhibited statistically significant (P < 0.05) metabolic differences comprising 27 different marker metabolites which showed highly significant values of VIP > 5 and P < 0.01. Results highlight differential regulation of 20 enzymatic genes that are involved in the biosynthesis of five different marker metabolites including acetaldehyde, homocysteine, cyclopropylamine, 1-pyrrolinium and halistanol sulfate.

CONCLUSIONS

The positive physiological effect of P. fortinii on R. crenulata encompasses differential regulation in carbohydrate metabolism, lipid metabolism and secondary metabolite synthesis. © 2020 Society of Chemical Industry.

摘要

背景

当红景天在低海拔地区培养时，其一些关键代谢物的含量往往会下降。有趣的是，人们发现内生真菌 Phialocephala fortinii 可以缓解这个问题。

结果

共鉴定到 16151 个差异基因，包括 14706 个上调和 1445 个下调的基因（P<0.05），共有 1432 种代谢物表现出统计学上显著的（P<0.05）代谢差异，包括 27 种不同的标记代谢物，它们的 VIP>5 和 P<0.01 值具有高度显著性。结果突出了参与五种不同标记代谢物生物合成的 20 个酶基因的差异调节，包括乙醛、同型半胱氨酸、环丙胺、1-吡咯啉和哈立斯坦醇硫酸盐。