Dellmann H D, Pearson C B
Stain Technol. 1977 Jan;52(1):5-8. doi: 10.3109/10520297709116736.
In the absence of other factors known to influence sectioning properties, high environmental relative humidity is shown to yield poorly embedded tissue. Humidity-related effects are avoided if the following embedding precedure is used; impregnate tissues using the following solutions 1) 70% alcohol - 5 minutes, 2) 95% alcohol - 2 x 15 minutes, 3) absolute alcohol - 3 x 20 minutes, 4) acetone - 2 x 15 minutes, 5) 1:1 mixture of acetone-epoxy resin (DDSA, 63.4 g; Araldite 502, 5.6 g; Epon 812, 39.4 g; DMP-30, 2.6 g) - 1 hour, 6) acetone-epoxy resin 1:3 - 1 hour, 7) epoxy resin - 1 hour; complete the preparation of blocks as follows 8) when tissues have been oriented in epoxy resin in flat embedding molds, place molds in one evacuated vacuum desiccator 10 cm above a 2 cm layer of Drierite for 24 hours at room temperature, 9) raise temperature to 60 C and maintain for 3 days to cure resin.
在不存在其他已知会影响切片性能的因素的情况下，高环境相对湿度会导致组织包埋效果不佳。如果采用以下包埋步骤，可避免与湿度相关的影响；使用以下溶液对组织进行浸渍：1）70%酒精 - 5分钟，2）95%酒精 - 2次，每次15分钟，3）无水酒精 - 3次，每次20分钟，4）丙酮 - 2次，每次15分钟，5）丙酮 - 环氧树脂（十二烷基琥珀酸酐，63.4克；阿乐得502，5.6克；Epon 812，39.4克；DMP - 30，2.6克）1:1混合物 - 1小时，6）丙酮 - 环氧树脂1:3 - 1小时，7）环氧树脂 - 1小时；按以下步骤完成包埋块的制备：8）当组织在平埋模具中用环氧树脂定向后，将模具放在一个抽空的真空干燥器中，在室温下，位于2厘米厚的无水氯化钙层上方10厘米处放置24小时，9）将温度升至60℃并保持3天以使树脂固化。
