Grechishnikov V I
Arkh Patol. 1981;43(3):76-7.
A method of embedding of biological objects for electron microscopy is described. A solution of epoxide resin ED-20 with a plastifier, dibutyl phthalate, and a solidifier, standard polyethylene polyamine, comprising 8% and 12% of the total mass of resin, was added into capsules into which blocks of tissues to be examined that had been pre-impregnated in increasing resin-in-acetone solution series were placed. Polymerization occurred under conventional conditions at room temperature for 24 hours or at 37 degrees C for 12 hours.
描述了一种用于电子显微镜检查的生物样本包埋方法。将含有增塑剂邻苯二甲酸二丁酯和固化剂标准聚乙烯多胺的环氧树脂ED-20溶液(分别占树脂总质量的8%和12%)加入胶囊中,在这些胶囊中放置已在浓度递增的树脂-丙酮溶液系列中预浸渍过的待检查组织块。聚合反应在常规条件下于室温进行24小时或在37℃下进行12小时。