Zhang Yao, Ye Qiang, Liu Bo, Feng Zhiping, Zhang Xian, Luo Mingyou, Yang Lijuan
College of Bioengineering, Sichuan University of Science & Engineering, Yinbin 643000, China.
Xufu Distillery Co. Ltd., Yibin 644000, China.
J Agric Food Chem. 2024 Apr 10;72(16):9259-67. doi: 10.1021/acs.jafc.3c08067.
The microbial consortium FA12 that can release ferulic acid (FA) by fermenting distiller's grains was screened from Daqu. , and were highly abundant in FA12 by 16S rRNA gene sequencing. In the process of long-term acclimation with distiller's grains as a medium, the biomass of FA12 remained stable, and the pH value of fermentation liquid was also relatively stable. Meanwhile, the activities of cellulase, xylanase, and feruloyl esterase secreted by FA12 were stable in the ranges of 0.2350-0.4470, 0.1917-0.3078, and 0.1103-0.1595 U/mL, respectively, and the release of FA could reach 133.77 μg/g. It is proven that the microbial consortium has good genetic stability. In addition, the structural changes of lignocellulose in distiller's grains before and after fermentation were analyzed by scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR), and the changes of distiller's grains weight and lignocellulose content before and after fermentation were also detected. These results all confirmed that FA12 had the function of degrading distiller's grains. In this study, we explored a method to use microbial communities to release FA from distiller's grains and degrade lignocellulose in the waste, which opened up a new way for the application of the high value of lost waste.
从大曲中筛选出能通过发酵酒糟释放阿魏酸（FA）的微生物群落FA12。通过16S rRNA基因测序发现， 、 在FA12中含量很高。在以酒糟为培养基的长期驯化过程中，FA12的生物量保持稳定，发酵液的pH值也相对稳定。同时，FA12分泌的纤维素酶、木聚糖酶和阿魏酸酯酶的活性分别稳定在0.2350 - 0.4470、0.1917 - 0.3078和0.1103 - 0.1595 U/mL范围内，FA的释放量可达133.77 μg/g。证明该微生物群落具有良好的遗传稳定性。此外，通过扫描电子显微镜（SEM）、X射线衍射（XRD）和傅里叶变换红外光谱（FTIR）分析了酒糟发酵前后木质纤维素的结构变化，并检测了酒糟发酵前后的重量和木质纤维素含量变化。这些结果均证实FA12具有降解酒糟的功能。在本研究中，我们探索了一种利用微生物群落从酒糟中释放FA并降解废弃物中木质纤维素的方法，为废弃物高值化利用开辟了新途径。
