Opt Express. 2020 May 11;28(10):15505-15514. doi: 10.1364/OE.390850.
We report a shot noise limited high-speed stimulated Raman microscopy platform allowing to acquire molecular vibrational spectra over 200 cm in 12 µs at a scan rate of 40kHz. Using spectral focusing together with optimized acousto-optics programmable dispersive filters, the designed low noise imaging platform performs chemical imaging of dynamical processes such as Mannitol crystal hydration and reaches a signal to noise ratio sufficient to perform label free histological imaging on frozen human colon tissue slides.
我们报告了一个受散粒噪声限制的高速受激拉曼显微镜平台，该平台允许在 40kHz 的扫描速率下在 12μs 内采集超过 200cm 的分子振动光谱。通过使用光谱聚焦和优化的声光可编程色散滤波器，设计的低噪声成像平台对曼尼托冰晶水合等动态过程进行化学成像，并实现了足以对冷冻人结肠组织切片进行无标记组织学成像的信噪比。
