Shi Lingyan, Fung Anthony A, Zhou Andy
Department of Bioengineering, University of California San Diego, La Jolla, CA, USA.
Quant Imaging Med Surg. 2021 Mar;11(3):1078-1101. doi: 10.21037/qims-20-712.
Stimulated Raman scattering (SRS) microscopy has emerged in the last decade as a powerful optical imaging technology with high chemical selectivity, speed, and subcellular resolution. Since the invention of SRS microscopy, it has been extensively employed in life science to study composition, structure, metabolism, development, and disease in biological systems. Applications of SRS in research and the clinic have generated new insights in many fields including neurobiology, tumor biology, developmental biology, metabolomics, pharmacokinetics, and more. Herein we review the advances and applications of SRS microscopy imaging in tissues and animals, as well as envision future applications and development of SRS imaging in life science and medicine.
受激拉曼散射（SRS）显微镜在过去十年中已成为一种强大的光学成像技术，具有高化学选择性、速度和亚细胞分辨率。自SRS显微镜发明以来，它已广泛应用于生命科学领域，用于研究生物系统中的组成、结构、代谢、发育和疾病。SRS在研究和临床中的应用在许多领域产生了新的见解，包括神经生物学、肿瘤生物学、发育生物学、代谢组学、药代动力学等等。在此，我们综述了SRS显微镜成像在组织和动物中的进展及应用，并展望了SRS成像在生命科学和医学中的未来应用与发展。
