Reproductive Center, Peking University Shenzhen Hospital, Shenzhen 518000, China.
Division of Reproductive Endocrinology and Infertility, Department of Obstetrics, Yale School of Medicine, Gynecology, and Reproductive Sciences, New Haven, Connecticut, 06512, USA.
Reprod Biol Endocrinol. 2020 May 13;18(1):46. doi: 10.1186/s12958-020-00578-z.
The H19 long noncoding RNA (lncRNA) belongs to a highly conserved, imprinted gene cluster involved in embryonic development and growth control. We previously described a novel mechanism whereby the Anti-mullerian hormone (Amh) appears to be regulated by H19. However, the relationship between circulating H19 and markers of ovarian reserve including AMH not been investigated.
To determine whether H19 expression is altered in women with decreased ovarian reserve.
Experimental study.
Yale School of Medicine (New Haven, USA) and Gazi University School of Medicine (Ankara, Turkey).
A total of 141 women undergoing infertility evaluation and treatment.
Collection of discarded blood samples and cumulus cells at the time of baseline infertility evaluation and transvaginal oocyte retrieval, respectively.
Serum and cumulus cell H19 expression.
Women with diminished ovarian reserve (as determined by AMH) had significantly lower serum H19 expression levels as compared to controls (p < 0.01). Serum H19 was moderately positively correlated with serum AMH. H19 expression was increased 3.7-fold in cumulus cells of IVF patients who demonstrated a high response to gonadotropins, compared to low responders (p < 0.05).
In this study, we show that downregulation of H19 in serum and cumulus cells is closely associated with decreased ovarian reserve, as measured by decreased AMH levels and reduced oocyte yield at oocyte retrieval. Further study with expanded sample sizes is necessary to determine whether H19 may be of use as a novel biomarker for diminished ovarian reserve.
H19 长非编码 RNA（lncRNA）属于高度保守的印记基因簇，参与胚胎发育和生长调控。我们之前描述了一种新的机制，即抗缪勒管激素（Amh）似乎受到 H19 的调控。然而，循环 H19 与包括 AMH 在内的卵巢储备标志物之间的关系尚未被研究。
确定 H19 表达是否在卵巢储备减少的女性中发生改变。
实验研究。
美国耶鲁大学医学院（纽黑文）和土耳其加济大学医学院（安卡拉）。
共 141 名接受不孕评估和治疗的女性。
分别在基线不孕评估时采集废弃的血液样本和卵丘细胞，以及经阴道取卵时采集卵丘细胞。
血清和卵丘细胞 H19 表达。
与对照组相比，卵巢储备减少（通过 AMH 确定）的女性血清 H19 表达水平显著降低（p<0.01）。血清 H19 与血清 AMH 呈中度正相关。与低反应者相比，在对促性腺激素反应良好的 IVF 患者的卵丘细胞中，H19 表达增加了 3.7 倍（p<0.05）。
在这项研究中，我们表明，血清和卵丘细胞中 H19 的下调与卵巢储备减少密切相关，这可以通过 AMH 水平降低和取卵时卵母细胞产量减少来衡量。需要进一步扩大样本量的研究来确定 H19 是否可用作卵巢储备减少的新型生物标志物。
