Tellez-Yudilevich M, Tyhurst M, Howell S L, Sheldon J
Transplantation. 1977 Mar;23(3):217-21. doi: 10.1097/00007890-197703000-00003.
Experiments were performed with rat pancreas to investigate optimum conditions for obtaining and preserving the pancreas for subsequent isolation of islets that were viable by both functional and morphological criteria. After only 30 min of warm ischaemia, the yield and viability of islets that could be isolated were poor. However, if the pancreas was removed and placed immediately in a cold bicarbonate-buffered medium, which was supplemented with HEPES to maintain the pH and Trasylol to inhibit proteolytic activity, then viable islets could be isolated consistently after over 8 hr of storage. These results demonstrate that even short periods of warm ischaemia will render the pancreas unsuitable for islet isolation. Once the pancreas is placed in a suitable cold medium, however, considerable delay may be permissible without adversely affecting the viability of the islets which can be obtained. The same conditions might prove to be applicable when human pancreas is obtained for the purpose of transplantation into diabetic subjects.
利用大鼠胰腺进行实验，以研究获取和保存胰腺的最佳条件，以便随后分离出在功能和形态学标准上均具有活力的胰岛。仅经过30分钟的热缺血后，能够分离出的胰岛产量和活力都很差。然而，如果将胰腺取出并立即置于冷的碳酸氢盐缓冲培养基中，该培养基添加了HEPES以维持pH值，并添加了抑肽酶以抑制蛋白水解活性，那么在储存超过8小时后仍能持续分离出有活力的胰岛。这些结果表明，即使是短时间的热缺血也会使胰腺不适用于胰岛分离。然而，一旦将胰腺置于合适的冷培养基中，则可以允许相当长的延迟时间，而不会对所获得的胰岛活力产生不利影响。当获取人类胰腺用于移植给糖尿病患者时，相同的条件可能也适用。
