Institute of Industrial Science, The University of Tokyo, 4-6-1, Komaba, Meguro-ku, 153-8505 Tokyo, Japan.
JST, PRESTO, 4-1-8 Honcho, Kawaguchi, 332-0012 Saitama, Japan.
ChemistryOpen. 2024 Oct;13(10):e202400098. doi: 10.1002/open.202400098. Epub 2024 Sep 5.
Spermidine is an essential biomarker related to antiaging. Although the detection of spermidine levels is in high demand in life science fields, easy-to-use analytical tools without sample purification have not yet been fully established. Herein, we propose an organic field-effect transistor-based chemical sensor for quantifying the spermidine concentration in commercial cosmetics. An extended-gate structure was employed for organic field-effect transistor (OFET)-based chemical sensing in aqueous media. A coordination-bond-based sensing system was introduced into the OFET device to visualize the spermidine detection information through changes in the transistor characteristics. The extended-gate-type OFET has shown quantitative responses to spermidine, which indicates sufficient detectability (i. e., the limit of detection for spermidine: 2.3 μM) considering actual concentrations in cosmetics. The applicability of the OFET-based chemical sensor for cosmetic analysis was validated by instrumental analysis using high-performance liquid chromatography. The estimated recovery rates for spermidine in cosmetic ingredient products (108-111 %) suggest the feasibility of cosmetic analysis based on the OFET-based chemical sensor.
精胺是一种与抗衰老相关的重要生物标志物。尽管在生命科学领域对精胺水平的检测需求很高，但尚未完全建立无需样品纯化的易用分析工具。在此，我们提出了一种基于有机场效应晶体管的化学传感器，用于定量检测商业化妆品中的精胺浓度。扩展门结构用于在水介质中进行基于有机场效应晶体管（OFET）的化学传感。将基于配位键的传感系统引入到 OFET 器件中，通过晶体管特性的变化来可视化精胺检测信息。扩展门型 OFET 对精胺表现出定量响应，这表明考虑到化妆品中的实际浓度，具有足够的检测能力（即，精胺的检测限：2.3 μM）。通过高效液相色谱的仪器分析验证了基于 OFET 的化学传感器在化妆品分析中的适用性。在化妆品成分产品中，精胺的估计回收率（108-111%）表明了基于 OFET 化学传感器进行化妆品分析的可行性。
