Ujigo Satoshi, Jonsson Daniel, Bogestål Yalda, Håkansson Joakim, Rosendahl Jennifer, Brive Lena, Olmarker Kjell
Musculoskeletal research, Department of Medical Chemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Department of Orthopaedic Surgery, National Hospital Organization Higashihiroshima Medical Center, Hiroshima, Japan.
Spine Surg Relat Res. 2019 Jul 10;4(2):117-123. doi: 10.22603/ssrr.2019-0010. eCollection 2020.
A disc herniation has traditionally been considered as disc tissue that has slipped out from an intervertebral disc. However, it was recently suggested that the disc herniation mass is a product of bioactive substances from the disc and that the disc hernia would more likely be scar tissue than herniated disc material. In this study, we aimed to analyze the structural components of experimentally induced disc herniations and compare with scar tissue and nucleus pulposus, in the rat.
Twenty-eight rats had their L4-5 discs punctured. After three weeks, the nodule that had been formed over the puncture site, scar tissue from the spine musculature, and normal nucleus pulposus were harvested and processed for further analysis.
Proteomics analysis demonstrated that the formed nodule was more similar to scar tissue than to nucleus pulposus. Gene expression analysis showed that there was no resemblance between any tissues when looking at inflammatory genes but that, there was a clear resemblance between the nodule and scar tissue when analyzing extracellular matrix-related genes. Analysis of the GAG and polysaccharide size distribution revealed that only the nodule and scar tissue contained the shorter versions, potentially short chain hyaluronic acid that is known to induce inflammatory responses. The hematoxylin and eosin stained sections of the nodule, disc tissue, and scar tissue indicated that the morphology of the nodule and scar tissue was very similar.
The nodule formed after experimental disc puncture, and that resembles a disc hernia, has a more structural resemblance to scar tissue than disc tissue. The nodule is, therefore, more likely to be induced by disc-derived bioactive substances than being formed by herniated disc material.
传统上，椎间盘突出被认为是椎间盘组织从椎间盘中脱出。然而，最近有研究表明，椎间盘突出块是椎间盘生物活性物质的产物，并且椎间盘疝更可能是瘢痕组织而非突出的椎间盘物质。在本研究中，我们旨在分析实验诱导的大鼠椎间盘突出的结构成分，并与瘢痕组织和髓核进行比较。
对28只大鼠的L4-5椎间盘进行穿刺。三周后，收集穿刺部位形成的结节、脊柱肌肉组织的瘢痕组织以及正常髓核，并进行进一步分析。
蛋白质组学分析表明，形成的结节与瘢痕组织的相似性高于与髓核的相似性。基因表达分析显示，在观察炎症基因时，任何组织之间都没有相似性，但在分析细胞外基质相关基因时，结节与瘢痕组织之间有明显的相似性。对糖胺聚糖和多糖大小分布的分析表明，只有结节和瘢痕组织含有较短的版本，可能是已知会诱导炎症反应的短链透明质酸。结节、椎间盘组织和瘢痕组织的苏木精和伊红染色切片表明，结节和瘢痕组织的形态非常相似。
实验性椎间盘穿刺后形成的类似椎间盘疝的结节，与瘢痕组织的结构相似性高于与椎间盘组织的相似性。因此，该结节更可能是由椎间盘衍生的生物活性物质诱导形成，而非由突出的椎间盘物质形成。
