Department of Orthopedic Surgery, The First Affiliated Hospital of Harbin Medical University, 150001, Harbin, China.
Department of Orthopedic Surgery, General Hospital of Heilongjiang Province Land Reclamation Headquarter, 150001, Harbin, China.
J Mater Sci Mater Med. 2020 May 13;31(5):48. doi: 10.1007/s10856-020-06384-8.
Skin injury is a kind of common tissue damage in daily life and war. Silk fibroin (SF) is becoming an engineered material for skin wound repair due to its superior unique physical and chemical properties. The present study aimed to illustrate mechanism of SF hydrogel promoting skin repair in the second degree burn mice.
Heat shock models were established. In vitro, cells were culture for 50 min at 44 °C water bath; while in vivo, the skin of anesthetic mice were treat with soldering iron at 90 °C. Then, they divided into silk fibroin gel group, purilon gel group and control (blank) group. The cellular activity of proliferation and apoptosis was detected by Kit-8, flow cytometry and HE-staining, and the migration and adhesion were detected by scratch test. qRT-PCR and WB were employed to detected adhesion and migration related genes and proteins expression. TLN1 siRNA and overexpression technologies were also employed to illustrate the potential mechanism of SF effects.
Compared with the purilon gel group and control group, SF hydrogel could enhance cell proliferation, migration and adhesion and increase the expression of adhesion and migration related proteins (P < 0.05), which promote burn wound healing.
Through the inhibition, overexpression and rescue experiments of Talin1, we proved that silk fibroin hydrogel promote burn wound healing through regulating TLN1 expression and affecting cell adhesion and migration.
皮肤损伤是日常生活和战争中常见的组织损伤。丝素蛋白（SF）由于其优异的独特物理和化学性质，正成为一种用于皮肤伤口修复的工程材料。本研究旨在阐明 SF 水凝胶促进二度烧伤小鼠皮肤修复的机制。
建立热休克模型。体外，将细胞在 44°C 的水浴中培养 50min；而在体内，用 90°C 的烙铁处理麻醉小鼠的皮肤。然后，将它们分为丝素蛋白凝胶组、普鲁兰凝胶组和对照组（空白）。通过 Kit-8、流式细胞术和 HE 染色检测细胞增殖和凋亡活性，通过划痕试验检测迁移和黏附。采用 qRT-PCR 和 WB 检测黏附和迁移相关基因和蛋白的表达。还采用 TLN1 siRNA 和过表达技术阐明 SF 作用的潜在机制。
与普鲁兰凝胶组和对照组相比，SF 水凝胶可增强细胞增殖、迁移和黏附，并增加黏附和迁移相关蛋白的表达（P<0.05），从而促进烧伤伤口愈合。
通过 Talin1 的抑制、过表达和挽救实验，我们证明丝素蛋白水凝胶通过调节 TLN1 表达并影响细胞黏附和迁移来促进烧伤伤口愈合。
