Katsikari Evrydiki, Kyriaki Alexandra, Vitsos Andreas, Vidali Margarita, Harizanis Paschalis, Sfiniadakis Ioannis, Kostaki Maria, Ieronymaki Dimitra, Terezaki Asimina, Ladopoulos Georgios, Albani Chara, Barda Christina, Rallis Michail Christou
Division of Pharmaceutical Technology, Department of Pharmacy, National and Kapodistrian University of Athens, 15784 Athens, Greece.
Laboratory of Sericulture & Apiculture, Agricultural University of Athens, 11855 Athens, Greece.
Medicines (Basel). 2025 Apr 30;12(2):11. doi: 10.3390/medicines12020011.
Burn injuries present significant treatment challenges due to the intricate nature of the healing process. L. (silkworm) derivatives, containing healing-promoting proteins such as sericin and fibroin, as well as the anti-inflammatory enzyme serrapeptase, have shown promise as potential healing agents. This study aimed to identify the optimal dosage of silkworm body and gland extracts for burn healing, compare the selected dose's effectiveness with that of silkworm cocoons, and assess the combined healing effects of a cocoon dressing and a silkworm body extract gel. An experimental model was employed using hairless SKH-hr2 female mice subjected to standardized second-degree burns. The mice received treatments with various formulations of silkworm body and gland extracts, silkworm cocoons, and a combined application of a cocoon dressing and silkworm body extract gel. The most effective treatments were the cocoon dressing and the combination of cocoon dressing with 60% body extract gel. By Day 20, complete healing (100%) was observed in the 20% and 60% body and gland extract groups, while the cocoon and 60% gland extract groups exhibited 60% healing, significantly higher than the control group (0% healing). Wound contraction analysis showed the greatest reduction in surface area from Day 3 to Day 17 in the cocoon and 60% body extract groups ( < 0.05). Histopathological assessments revealed that the combination group exhibited the least tissue damage (score: 7), compared to the control (score: 10-13). The study highlights the poorly examined therapeutic potential of silkworm body and gland extracts, demonstrating their efficacy in accelerating burn healing. The effects observed by the silkworm cocoon and body extract suggests a novel and promising approach for burn wound management, warranting further clinical exploration.
由于愈合过程的复杂性，烧伤治疗面临重大挑战。家蚕衍生物含有促进愈合的蛋白质，如丝胶蛋白和丝素蛋白，以及抗炎酶丝氨酸蛋白酶，已显示出作为潜在愈合剂的前景。本研究旨在确定家蚕体和腺体提取物促进烧伤愈合的最佳剂量，将所选剂量的有效性与家蚕茧进行比较，并评估茧敷料和家蚕体提取物凝胶的联合愈合效果。使用无毛SKH-hr2雌性小鼠建立标准化二度烧伤的实验模型。小鼠接受了各种家蚕体和腺体提取物配方、家蚕茧以及茧敷料和家蚕体提取物凝胶联合应用的治疗。最有效的治疗方法是茧敷料以及茧敷料与60%体提取物凝胶的组合。到第20天，20%和60%体和腺体提取物组观察到完全愈合（100%），而茧和60%腺体提取物组的愈合率为60%，显著高于对照组（0%愈合）。伤口收缩分析显示，茧和60%体提取物组从第3天到第17天表面积减少最大（<0.05）。组织病理学评估显示，与对照组（评分：10 - 13）相比，联合组的组织损伤最小（评分：7）。该研究突出了家蚕体和腺体提取物尚未充分研究的治疗潜力，证明了它们在加速烧伤愈合方面的功效。家蚕茧和体提取物观察到的效果为烧伤创面管理提出了一种新颖且有前景的方法，值得进一步临床探索。
