Department of Encephalopathy II, Rizhao Hospital of Traditional Chinese Medicine, Rizhao, China.
Ju County Hospital of Traditional Chinese Medicine, Rizhao, China -
Minerva Med. 2020 Dec;111(6):544-550. doi: 10.23736/S0026-4806.20.06532-5. Epub 2020 May 13.
The aim of this study is to investigate the expression and clinical value of miRNA-128 (miR-128) and insulin-like growth factor-1 (IGF-1) in patients with acute ischemic stroke (AIS).
Eighty patients with acute ischemic stroke hospitalized in Rizhao Hospital of TCM from August 2016 to August 2018 were selected as the experimental group. Sixty healthy patients with normal physical examination during the same period were selected as the control group. The expression levels of miR-128 and IGF-1 were compared between the two groups and between AIS patients with different levels of nervous functional defects, different infarct size and different prognosis. ROC curve of serum miR-128 and IGF-1 expressions for AIS diagnosis alone and in combination was analyzed.
The expression level of miR-128 in the serum of patients in the experimental group was higher than that of the control group, and the expression level of IGF-1 was lower than that of the control group (P<0.001). The serum miR-128 expression of patients in the ineffective group was higher than that of the effective group, and the expression of IGF-1 was lower than that of the effective group (P<0.001). The expression level of serum miR-128 in AIS patients was higher than that in healthy subjects and increased with the aggravation of the disease. The expression level of IGF-1 of AIS patients was lower than that of healthy subjects and decreased with the aggravation of the disease. The two factors were involved in the formation and deterioration of the disease.
The expression levels can reflect the severity of the disease to a certain extent and provide references for the early diagnosis and prognostic treatment in patients with acute ischemic stroke.
本研究旨在探讨微小 RNA-128（miR-128）和胰岛素样生长因子-1（IGF-1）在急性缺血性脑卒中（AIS）患者中的表达及临床价值。
选取 2016 年 8 月至 2018 年 8 月日照市中医医院收治的急性缺血性脑卒中患者 80 例作为实验组，同期选取 60 例健康体检者作为对照组。比较两组及不同神经功能缺损程度、不同梗死面积、不同预后 AIS 患者的 miR-128 和 IGF-1 表达水平。分析血清 miR-128 和 IGF-1 单独及联合表达对 AIS 的诊断 ROC 曲线。
实验组患者血清 miR-128 表达水平高于对照组，IGF-1 表达水平低于对照组（P<0.001）。无效组患者血清 miR-128 表达高于有效组，IGF-1 表达低于有效组（P<0.001）。AIS 患者血清 miR-128 表达水平高于健康人群，且随病情加重而升高，IGF-1 表达水平低于健康人群，且随病情加重而降低。两因素均参与疾病的发生发展。
表达水平在一定程度上可反映病情严重程度，可为急性缺血性脑卒中患者的早期诊断和预后治疗提供参考。
