Neurorehabilitation Center, Beijing Rehabilitation Hospital of Capital Medical University, Beijing 100144, China.
Department of Encephalopathy, Binzhou Hospital of Traditional Chinese Medicine, Binzhou, Shandong 256600, China.
Clinics (Sao Paulo). 2021 Oct 29;76:e2958. doi: 10.6061/clinics/2021/e2958. eCollection 2021.
To investigate the clinical utility of serum microRNA levels (miR-9-5p and miR-128-3p) in the diagnosis and prognosis of early-stage acute ischemic stroke (AIS).
We compared the differences in serum miR-9-5p and miR-128-3p levels between patients with AIS and healthy individuals (controls). The serum levels of miR-9-5p and miR-128-3p were quantified using quantitative real-time PCR, and the association of each miRNA with AIS was determined using receiver operator characteristic curve analysis. The predictive value of these indices in the diagnosis of early-stage AIS was evaluated in conjunction with that of computed tomography findings and neuron-specific enolase levels. The prognosis of patients with AIS was evaluated three months after their discharge from hospital using the modified Rankin scale, which classifies the prognosis as either favorable or poor. Logistic regression analysis was used to analyze the correlation between miR-9-5p and miR-128-3p levels and patient prognosis.
The serum levels of miR-9-5p and miR-128-3p were upregulated in patients with AIS relative to those in healthy individuals. A pronounced correlation was identified between serum miR-9-5p and miR-128-3p levels and patient prognosis, with high levels of both miRNAs being associated with poor patient outcomes.
Assessment of serum miR-9-5p and miR-128-3p levels is important for the early diagnosis and prognosis of AIS.
探究血清 microRNA 水平（miR-9-5p 和 miR-128-3p）在早期急性缺血性脑卒中（AIS）诊断和预后中的临床应用价值。
我们比较了 AIS 患者和健康个体（对照组）之间血清 miR-9-5p 和 miR-128-3p 水平的差异。采用实时定量 PCR 定量检测 miR-9-5p 和 miR-128-3p 的血清水平，通过受试者工作特征曲线分析确定每个 miRNA 与 AIS 的相关性。结合计算机断层扫描（CT）结果和神经元特异性烯醇化酶（NSE）水平，评估这些指标在早期 AIS 诊断中的预测价值。采用改良 Rankin 量表评估 AIS 患者出院后 3 个月的预后，将预后分为良好和不良。采用 logistic 回归分析分析 miR-9-5p 和 miR-128-3p 水平与患者预后的相关性。
与健康个体相比，AIS 患者的血清 miR-9-5p 和 miR-128-3p 水平升高。血清 miR-9-5p 和 miR-128-3p 水平与患者预后呈显著相关，高水平的两种 miRNA 与患者不良预后相关。
评估血清 miR-9-5p 和 miR-128-3p 水平对 AIS 的早期诊断和预后具有重要意义。
