Department of Veterinary Hygiene and Public Health, São Paulo State University (Unesp), School of Veterinary Medicine and Animal Science, Botucatu, São Paulo State, Brazil.
Laboratti Veterinary Diagnostic Laboratory, Pedrinhas Paulista, São Paulo State, Brazil.
J Dairy Res. 2020 Aug;87(3):306-308. doi: 10.1017/S0022029920000205. Epub 2020 May 14.
The work reported in this research communication investigated the occurrence of Mycoplasma bovis (M. bovis) in milk samples from cows with clinical mastitis on dairy farms from seven Brazilian states. We hypothesized that M. bovis was present in bovine clinical mastitis milk in Brazil. A total of 561 milk samples were cultured on Hayflick agar and incubated in a microaerophilic atmosphere at 5% CO2. Polymerase chain reaction (PCR) was performed for the detection of Mycoplasma spp. and Mycoplasma bovis in milk samples. Mycoplasma spp. were isolated in 2% of the milk samples, and Mycoplasma bovis was verified in 3% of the milk samples by PCR. The results showed that Mycoplasma bovis is involved in clinical mastitis in Brazilian dairy herds. We emphasize the need for further studies to investigate the infection by this agent in clinical mastitis cases, particularly in Brazil, due to the lack of knowledge about its prevalence.
本研究通讯报道的工作调查了来自巴西七个州的奶牛场患有临床乳腺炎的奶牛的牛奶样本中牛支原体（M. bovis）的发生情况。我们假设 M. bovis 存在于巴西牛的临床乳腺炎牛奶中。总共培养了 561 份牛奶样本在 Hayflick 琼脂上，并在 5%CO2 的微需氧环境中孵育。对牛奶样本进行聚合酶链反应（PCR）以检测支原体属和牛支原体。在 2%的牛奶样本中分离出支原体属，在 3%的牛奶样本中通过 PCR 验证了牛支原体。结果表明，牛支原体参与了巴西奶牛群的临床乳腺炎。我们强调需要进一步研究来调查该病原体在临床乳腺炎病例中的感染情况，特别是在巴西，因为对其流行率知之甚少。
