乙醇叶提取物对环磷酰胺诱导的大鼠贫血的抗贫血作用。

Anti-anaemic effect of ethanol leaf extract of on cyclophosphomide-induced anaemia in rats.

机构信息

Department of Veterinary Pathology, University of Ilorin, Ilorin, Nigeria.

Faculty of Veterinary Medicine and Surgery, University of Ilorin, Ilorin, Nigeria.

出版信息

J Complement Integr Med. 2020 May 15;18(1):87-92. doi: 10.1515/jcim-2020-0006.

DOI:10.1515/jcim-2020-0006

PMID:32406386

Abstract

BACKGROUND

The folkloric claim that (Chaya) could ameliorate anaemia requires scientific revalidation in anaemic models.

OBJECTIVE

The aim of this study is to investigate the anti-anaemic effect of on cyclophosphamide-induced anaemia in rats.

METHODS

The leaves of were collected, dried and extracted with ethanol. Twenty five Wistar Albino rats weighing 120-180 kg were used. Anaemia was induced in groups 2, 3, 4 and 5 with a single injection of cyclophosphamide, while group 1 served as a negative control without anemia and was treated with 0.5 mL of normal saline, Group 2 rats were treated with 100 mg/kg body weight of Group 3 rats were treated with 500 mg/kg body weight of Group 4 served as the positive control rats and was treated with 0.5 mL of normal saline. Group 5 which served as the standard control rats and were treated with 5 mg/kg body weight of standard drug Chemiron. The treated lasted for two weeks during which blood samples were collected from each rat for haematological analysis.

RESULTS

The result shows that hematological parameters were elevated in groups 2, 3 and 5 rats treated with 100 mg/kg and 500 mg/kg body weight of ethanol extract of and chemiron respectively.

CONCLUSIONS

Ethanol leaf extract of at a graded dose of 500 mg/kg body weight had higher ameliorative effect on the haematological parameters of cyclophosphamide-induced anemia in rats.

摘要

背景

民间传说查雅（Chaya）可改善贫血，需要在贫血模型中进行科学验证。

目的

本研究旨在探讨查雅叶乙醇提取物对环磷酰胺诱导的大鼠贫血的抗贫血作用。

方法

采集、干燥并以乙醇提取查雅叶，使用 25 只体重为 120-180 克的 Wistar 白化大鼠。第 2、3、4 和 5 组大鼠一次性注射环磷酰胺诱导贫血，第 1 组作为无贫血的阴性对照，给予 0.5 毫升生理盐水，第 2 组大鼠给予 100 毫克/千克体重的查雅叶乙醇提取物，第 3 组大鼠给予 500 毫克/千克体重的查雅叶乙醇提取物，第 4 组作为阳性对照大鼠，给予 0.5 毫升生理盐水，第 5 组作为标准对照大鼠，给予 5 毫克/千克体重的标准药物 Chemiron。治疗持续两周，在此期间从每只大鼠收集血液样本进行血液学分析。