Department of Clinical Sciences/ENT, Umeå University, Umeå, Sweden.
Department of Medical Biosciences/Pathology, Umeå University, Umeå, Sweden.
Oral Dis. 2020 Oct;26(7):1414-1423. doi: 10.1111/odi.13414. Epub 2020 Jul 6.
To use alternative quantitation approaches to clarify the clinical implication of programmed cell death ligand 1 (PD-L1) in squamous cell carcinoma of the oral tongue (SCCOT).
Ventana SP263 immunohistochemistry assay and a multiplicative QuickScore method were applied to quantify PD-L1 in tumor and surrounding immune cells from 101 patients with SCCOT. Tumor-infiltrating immune cells were estimated from bulk tissue transcriptional profiles of 25 patients. Circulating PD-L1 levels were measured in serum from 30 patients using an electrochemiluminescence assay platform.
We found higher tumor cell PD-L1 levels in females than males (p = .019). For patients with low PD-L1 in tumor cells, better survival was seen in males than females (overall survival p = .021, disease-free survival p = .020). Tumor-infiltrating natural killer T cells, immature dendritic cells, and M1 macrophages were positively associated with tumor cell PD-L1 (p < .05).
Our data confirmed the significance of gender on tumor cell PD-L1 expression and demonstrated combined effects of gender and PD-L1 levels on clinical outcome in patients with SCCOT. The data also indicated the involvement of specific immune cell types in PD-L1-regulated immune evasion.
采用替代定量方法来阐明程序性死亡配体 1（PD-L1）在口腔舌鳞癌（SCCOT）中的临床意义。
对 101 例 SCCOT 患者的肿瘤和周围免疫细胞应用 Ventana SP263 免疫组化检测和乘法 QuickScore 方法进行 PD-L1 定量，从 25 例患者的肿瘤转录组学特征中评估肿瘤浸润免疫细胞，使用电化学发光检测平台测量 30 例患者血清中的循环 PD-L1 水平。
我们发现女性肿瘤细胞 PD-L1 水平高于男性（p =.019）。对于肿瘤细胞 PD-L1 低表达的患者，男性的生存状况优于女性（总生存 p =.021，无病生存 p =.020）。肿瘤浸润自然杀伤 T 细胞、未成熟树突状细胞和 M1 巨噬细胞与肿瘤细胞 PD-L1 呈正相关（p <.05）。
本研究数据证实了性别对肿瘤细胞 PD-L1 表达的意义，并证明了性别和 PD-L1 水平对 SCCOT 患者临床结局的综合影响。数据还表明，特定免疫细胞类型参与了 PD-L1 调节的免疫逃逸。
