Centre for Biospectroscopy and School of Chemistry, Monash University, Clayton Campus, Clayton 3800, Victoria, Australia.
Infection and Immunity Program, Monash Biomedicine Discovery Institute and Department of Microbiology, Monash University, Clayton Campus, Clayton 3800, Victoria, Australia.
Anal Chem. 2020 Jun 16;92(12):8235-8243. doi: 10.1021/acs.analchem.0c00474. Epub 2020 May 29.
Here, we applied vibrational spectroscopy to investigate the drug response following incubation of with oxacillin. The main focus of this work was to identify the chemical changes caused by oxacillin over time and to determine the feasibility of the spectroscopic approach to detect antimicrobial resistance. The oxacillin-induced changes in the chemical composition of susceptible bacteria, preceding (and leading to) the inhibition of growth, included an increase in the relative content of nucleic acids, alteration in the α-helical/β-sheet protein ratio, structural changes in carbohydrates (observed via changes in the band at 1035 cm), and significant thickening of the cell wall. These observations enabled a dose-dependent discrimination between susceptible bacteria incubated with and without oxacillin after 120 min. In methicillin resistant strains, no spectral differences were observed between cells, regardless of drug exposure. These results pave the way for a new, rapid spectroscopic approach to detect drug resistance in pathogens, based on their early positive/negative drug response.
在这里，我们应用振动光谱学研究了[具体细菌]与苯唑西林孵育后的药物反应。这项工作的主要重点是确定苯唑西林随时间引起的化学变化，并确定光谱方法检测抗微生物药物耐药性的可行性。在生长受到抑制之前和之后，苯唑西林引起的敏感细菌化学成分的变化包括核酸相对含量增加、α-螺旋/β-折叠蛋白比例改变、碳水化合物结构变化（通过 1035 cm 处的波段变化观察到）以及细胞壁显著增厚。这些观察结果使我们能够在 120 分钟后对与苯唑西林孵育的和未孵育的敏感细菌进行剂量依赖性区分。在耐甲氧西林的菌株中，无论药物暴露与否，细胞之间均未观察到光谱差异。这些结果为基于病原体早期阳性/阴性药物反应的新的快速光谱方法检测药物耐药性铺平了道路。
