Verma Awadhesh Kumar, Lakshmi G B V S, Dhiman Tarun Kumar, Hashmi S Z H, Kumar Anil, Solanki Pratima R
Special Centre for Nanoscience, Jawaharlal Nehru University, New Delhi, 110067, India.
School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, Punjab, 144001, India.
Sci Rep. 2025 Jan 10;15(1):1648. doi: 10.1038/s41598-024-62827-0.
Excess consumption of antibiotics leads to antibiotic resistance that hinders the control and cure of microbial diseases. Therefore, it is crucial to monitor the antibiotic levels in the environment. In this proposed research work, an optical nano-sensor was devised that can sense the ultra-low concentration of antibiotics, in samples like tap water using fluorescent zinc oxide quantum dots (ZnO QDs) based nano-sensor. For this, different polymers (polyvinylalcohol-PVA and polyvinylpyrrolidine-PVP) capped florescent ZnO QDs were synthesized using a modified sol-gel technique. These were used as fluorescent probes to monitor the presence of antibiotics. The optical characterizations of synthesized QDs were performed using UV-visible absorption and fluorescence spectroscopic methods while structural characteristics were analyzed by using Raman spectroscopy and X-ray diffraction spectroscopy. The formation of capped QDs was confirmed by Fourier transform infrared spectroscopy (FTIR). Charge on the synthesized QDs was obtained with the help of ZETA potential. Here ten different antibiotics were checked, Ciprofloxacin and Moxifloxacin have shown excellent sensing and specificity with PVA-ZnO QDs and PVP-ZnO QDs with LOD of 1.4 nM and 0.8 nM, and sensitivity of 36.17 units/mM and 19.33 units/mM respectively. This study also inferred the tuning of the ZnO QDs properties and specificity towards the different antibiotics can be achieved by capping QDs with different polymers.
抗生素的过度使用会导致抗生素耐药性，从而阻碍微生物疾病的控制和治愈。因此，监测环境中的抗生素水平至关重要。在这项拟开展的研究工作中，设计了一种光学纳米传感器，它可以使用基于荧光氧化锌量子点（ZnO QDs）的纳米传感器，检测诸如自来水等样品中超低浓度的抗生素。为此，采用改进的溶胶 - 凝胶技术合成了不同聚合物（聚乙烯醇 - PVA和聚乙烯吡咯烷酮 - PVP）包覆的荧光ZnO QDs。这些被用作荧光探针来监测抗生素的存在。使用紫外 - 可见吸收光谱和荧光光谱方法对合成的量子点进行光学表征，同时通过拉曼光谱和X射线衍射光谱分析其结构特征。通过傅里叶变换红外光谱（FTIR）确认了包覆量子点的形成。借助ZETA电位获得合成量子点的电荷。在此检测了十种不同的抗生素，环丙沙星和莫西沙星对PVA - ZnO QDs和PVP - ZnO QDs表现出优异的传感性能和特异性，检测限分别为1.4 nM和0.8 nM，灵敏度分别为36.17单位/ mM和19.33单位/ mM。该研究还推断，通过用不同聚合物包覆量子点，可以实现ZnO量子点性质的调节及其对不同抗生素的特异性。
