Crop Production and Pest Control Research Unit, Agricultural Research Service, USDA, and College of Agriculture, Purdue University, West Lafayette, Indiana, United States of America.
PLoS One. 2020 May 14;15(5):e0231296. doi: 10.1371/journal.pone.0231296. eCollection 2020.
Phytophthora sansomeana infects soybean and causes root rot. It was recently separated from the species complex P. megasperma sensu lato. In this study, we sequenced and annotated its complete mitochondrial genome and compared it to that of nine other Phytophthora species. The genome was assembled into a circular molecule of 39,618 bp with a 22.03% G+C content. Forty-two protein coding genes, 25 tRNA genes and two rRNA genes were annotated in this genome. The protein coding genes include 14 genes in the respiratory complexes, four ATP synthase genes, 16 ribosomal proteins genes, a tatC translocase gene, six conserved ORFs and a unique orf402. The tRNA genes encode tRNAs for 19 amino acids. Comparison among mitochondrial genomes of 10 Phytophthora species revealed three inversions, each covering multiple genes. These genomes were conserved in gene content with few exceptions. A 3' truncated atp9 gene was found in P. nicotianae. All 10 Phytophthora species, as well as other oomycetes and stramenopiles, lacked tRNA genes for threonine in their mitochondria. Phylogenomic analysis using the mitochondrial genomes supported or enhanced previous findings of the phylogeny of Phytophthora spp.
桑氏腐霉侵染大豆并引发根腐病。它最近从广义的大茎点霉种复合体中分离出来。在这项研究中，我们对其完整的线粒体基因组进行了测序和注释，并将其与其他 9 种腐霉物种的基因组进行了比较。基因组被组装成一个 39618bp 的圆形分子，G+C 含量为 22.03%。在这个基因组中注释了 42 个蛋白质编码基因、25 个 tRNA 基因和两个 rRNA 基因。蛋白质编码基因包括呼吸复合物中的 14 个基因、4 个 ATP 合酶基因、16 个核糖体蛋白基因、tatC 转运酶基因、6 个保守 ORF 和一个独特的 orf402。tRNA 基因编码用于 19 种氨基酸的 tRNA。对 10 种腐霉物种的线粒体基因组进行比较发现了三个倒位，每个倒位都覆盖了多个基因。这些基因组在基因内容上是保守的，只有少数例外。在烟草疫霉中发现了一个 3' 截断的 atp9 基因。所有 10 种腐霉物种以及其他卵菌和不等鞭毛类都缺乏线粒体中苏氨酸的 tRNA 基因。使用线粒体基因组进行的系统基因组分析支持或增强了先前对腐霉属物种系统发育的研究结果。
