Department of Plastic Surgery and Burn Center, Second Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, China.
Research Center of Translational Medicine, Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China.
Mech Dev. 2020 Jun;162:103609. doi: 10.1016/j.mod.2020.103609. Epub 2020 May 11.
Recent studies have shown that lipid metabolism was abnormal during the formation of cleft palate. However, the composition of these lipid species remains unclear.
Aims of this study were to identify the lipid species components and reveal the key lipid metabolic disorders in cleft palate formation.
The pregnant mice were divided into experimental group exposed to all-trans retinoic acid (RA-treated group) (n = 12) and control group (n = 12) at embryonic gestation day 10.5 (E0.5). The component of the palatal tissue metabolome was analyzed using a LCMS-based nontargeted lipidomics approach. Multivariate statistical analysis was then carried out to assess the differences between the RA-treated group and the control group.
Twenty-nine lipid species were found to discriminate between RA-treated and control embryos. Among them, 28 lipid species increased and 1 lipid species decreased in the RA-treated group. Among these lipids, 13 were triglycerides, 9 were PEs, 3 were PCs, 2 were PSs, 2 were DGs. Further analysis of the number of carbons and unsaturated bond of triglycerides showed that TGs with high unsaturated bonds constituted a higher fraction in the RA-treated group. A higher amount of triglycerides containing 52, 54, 56, 58, 60 carbons, and 1 to 8 unsaturated bonds. Of note, under RA treatment, TG 50:1, 52:2, 56:6and 60:8 became the most prominent.
Lipid metabolism is significantly different in the formation of cleft palate induced by RA, and the unsaturated triglycerides increased in the RA-treated group may play an important role in the formation of cleft palate.
最近的研究表明，在腭裂形成过程中脂质代谢异常。然而，这些脂质种类的组成仍不清楚。
本研究旨在鉴定脂质种类成分，揭示腭裂形成中关键的脂质代谢紊乱。
将妊娠第 10.5 天（E0.5）的孕鼠分为实验组（全反式视黄酸处理组）（n=12）和对照组（n=12）。采用基于 LCMS 的非靶向脂质组学方法分析腭组织代谢组的成分。然后进行多变量统计分析，以评估 RA 处理组与对照组之间的差异。
发现 29 种脂质种类可区分 RA 处理和对照胚胎。其中，28 种脂质种类在 RA 处理组中增加，1 种脂质种类减少。在这些脂质中，13 种是三酰甘油，9 种是 PEs，3 种是 PCs，2 种是 PSs，2 种是 DGs。进一步分析三酰甘油的碳原子数和不饱和键数表明，RA 处理组中具有较高不饱和键的三酰甘油构成更高的比例。具有较高数量的含有 52、54、56、58、60 个碳原子和 1 至 8 个不饱和键的三酰甘油。值得注意的是，在 RA 处理下，TG 50:1、52:2、56:6 和 60:8 变得最为突出。
RA 诱导腭裂形成过程中的脂质代谢明显不同，RA 处理组中增加的不饱和三酰甘油可能在腭裂形成中发挥重要作用。
