Miyaji S, Kamiya M, Shikata J
First Department of Surgery, Teikyo University School of Medicine, Tokyo, Japan.
Jikken Dobutsu. 1988 Oct;37(4):399-404.
The ovicidal effects of heat and various chemical disinfectants on an oxyurid rat nematode Syphacia muris were investigated, using the hatching methods in artificial intestinal juice. The eggs were collected from the perianal skin of spontaneously infected rats by means of a piece of transparent adhesive tapes, and these eggs were treated with each disinfectant for two hours. It was found that 70% ethanol and 80 degrees C 30 min treatments killed almost all of the eggs. However, a small number of the eggs tested was killed by 0.02% chlorhexidine digluconate or 0.05% benzethonium chloride. Alcide, 3% saponated cresol solution, 50% isopropanol, 10 ppm sodium hypochlorite and 5 ppm iodophol had some effects against the eggs, but they didn't kill the eggs completely. A biological assay through infection of the eggs to rats might be necessary because the effects of 2% formalin on the eggs were not determined by the hatching methods.
利用人工肠液中的孵化方法，研究了热和各种化学消毒剂对大鼠尖尾线虫——鼠管状线虫的杀卵效果。通过一片透明胶带从自然感染大鼠的肛周皮肤收集虫卵，并用每种消毒剂处理这些虫卵两小时。结果发现，70%乙醇和80℃处理30分钟几乎能杀死所有虫卵。然而，少量受试虫卵可被0.02%葡萄糖酸氯己定或0.05%苄索氯铵杀死。爱尔施、3%皂化甲酚溶液、50%异丙醇、10 ppm次氯酸钠和5 ppm碘伏对虫卵有一定作用，但不能完全杀死虫卵。由于2%福尔马林对虫卵的作用无法通过孵化方法确定，可能需要通过将虫卵感染大鼠进行生物测定。
