European Molecular Biology Laboratory (EMBL), Developmental Biology Unit, Meyerhofstrasse 1, 69117, Heidelberg, Germany.
European Molecular Biology Laboratory (EMBL), Developmental Biology Unit, Meyerhofstrasse 1, 69117, Heidelberg, Germany.
Curr Opin Cell Biol. 2020 Oct;66:19-27. doi: 10.1016/j.ceb.2020.04.004. Epub 2020 May 11.
Morphogenesis of multicellular systems is governed by precise spatiotemporal regulation of biochemical reactions and mechanical forces which together with environmental conditions determine the development of complex organisms. Current efforts in the field aim at decoding the system-level principles underlying the regulation of developmental processes. Toward this goal, optogenetics, the science of regulation of protein function with light, is emerging as a powerful new tool to quantitatively perturb protein function in vivo with unprecedented precision in space and time. In this review, we provide an overview of how optogenetics is helping to address system-level questions of multicellular morphogenesis and discuss future directions.
多细胞系统的形态发生受生化反应和机械力的精确时空调控的支配，这些因素与环境条件一起决定了复杂生物体的发育。该领域目前的努力旨在解码调控发育过程的系统级原理。为此，光遗传学——用光调控蛋白质功能的科学——正在成为一种强大的新工具，可前所未有地精确在空间和时间上定量扰乱体内蛋白质的功能。在这篇综述中，我们概述了光遗传学如何帮助解决多细胞形态发生的系统级问题，并讨论了未来的方向。
