Chen I T, Roufa D J
Center for Basic Cancer Research, Kansas State University, Manhattan 66506.
Gene. 1988 Oct 15;70(1):107-16. doi: 10.1016/0378-1119(88)90109-6.
A human ribosomal protein S17 cDNA [Chen et al., Proc. Natl. Acad. Sci. USA 83 (1986) 6907-6911] was used to isolate four S17 DNA clones from human genomic libraries constructed in bacteriophage lambda and cosmid vectors. Based on its transcriptional activity in a transient expression assay and on sequence similarity with S17 cDNA, cosmid clone HGS17-6 was identified as carrying the functional RPS17 gene. RPS17 is composed of five exons and four introns that span 4 kb of DNA. Two lambda clones of human genomic DNA were recognized as containing processed S17 pseudogenes, because (i) they were transcriptionally inactive in the transient assay, and (ii) they possess multiple, perfectly spliced RPS17 exons. Their coding sequences differ slightly from the cDNA and functional genomic clone.
一个人类核糖体蛋白 S17 cDNA [Chen 等人，《美国国家科学院院刊》83 (1986) 6907 - 6911] 被用于从构建于噬菌体 λ 和黏粒载体的人类基因组文库中分离出四个 S17 DNA 克隆。基于其在瞬时表达试验中的转录活性以及与 S17 cDNA 的序列相似性，黏粒克隆 HGS17 - 6 被鉴定为携带功能性 RPS17 基因。RPS17 由五个外显子和四个内含子组成，跨越 4 kb 的 DNA。两个人类基因组 DNA 的 λ 克隆被认为含有加工后的 S17 假基因，原因如下：(i) 它们在瞬时试验中无转录活性；(ii) 它们拥有多个完美剪接的 RPS17 外显子。它们的编码序列与 cDNA 和功能性基因组克隆略有不同。
