Centre for Functional and Surface Functionalized Glass, TnU AD, Trenčín, Slovakia; Institute of Biomaterials, University of Erlangen-Nuremberg, Erlangen, Germany.
Centre for Functional and Surface Functionalized Glass, TnU AD, Trenčín, Slovakia; Institute of Biomaterials, University of Erlangen-Nuremberg, Erlangen, Germany.
Mater Sci Eng C Mater Biol Appl. 2020 Jul;112:110909. doi: 10.1016/j.msec.2020.110909. Epub 2020 Mar 31.
Many elements, such as copper, cobalt (Co), strontium, magnesium and boron (B) have been used for single or multiple doping of bioactive glasses to promote angiogenesis during bone regeneration. However, few works have focused on promoting angiogenesis through stimulating several different signalling pathways which can be called a multi-target approach. In this study, 45S5 bioactive glass (BG) co-doped with B and Co was prepared by conventional melt quenching method. The synergistic effect of the two co-doping elements on angiogenesis promotion was expected. ATR-Fourier transform infrared spectroscopy, energy-dispersive X-ray spectroscopy and inductively coupled plasma-optical emission spectrometry were used to characterize the composition, element distribution and ion release of the samples, respectively. Results showed that the compositions of all samples were consistent with the design compositions and all elements were homogeneously distributed in the bulk glass. Different contents of B and Co led to different release rates of these elements. All samples showed bioactivity after immersion in simulated body fluid, regardless of the amount of doped B and Co. The 1% and 0.1% extracts of all samples did not show any cytotoxicity to MG-63 and ST-2 cells. Compared with single B or Co doping, the B and Co dual doped sample led to a stronger increase of the secretion of vascular endothelial growth factor from ST-2 cells, which supports and confirms the synergistic effect on angiogenesis of B and Co as co-doping elements in 45S5 BG.
许多元素，如铜、钴（Co）、锶、镁和硼（B），已被用于生物活性玻璃的单一或多种掺杂，以促进骨再生过程中的血管生成。然而，很少有研究关注通过刺激几种不同的信号通路来促进血管生成，这可以称为多靶点方法。在这项研究中，采用传统的熔融淬火法制备了 B 和 Co 共掺杂的 45S5 生物活性玻璃（BG）。期望这两种共掺杂元素在促进血管生成方面发挥协同作用。衰减全反射傅里叶变换红外光谱、能量色散 X 射线光谱和电感耦合等离子体-光学发射光谱分别用于表征样品的组成、元素分布和离子释放。结果表明，所有样品的组成均与设计组成一致，所有元素在块状玻璃中均匀分布。不同含量的 B 和 Co 导致这些元素的释放速率不同。所有样品在模拟体液中浸泡后均表现出生物活性，无论掺杂的 B 和 Co 含量多少。所有样品的 1%和 0.1%浸提液对 MG-63 和 ST-2 细胞均无细胞毒性。与单一 B 或 Co 掺杂相比，B 和 Co 双掺杂样品导致 ST-2 细胞分泌的血管内皮生长因子增加更为明显，这支持并证实了 B 和 Co 作为 45S5 BG 共掺杂元素在促进血管生成方面的协同作用。
