College of Environment, IT & Engineering, Charles Darwin University, Darwin 0909, Australia.
College of Environment, IT & Engineering, Charles Darwin University, Darwin 0909, Australia.
Mater Sci Eng C Mater Biol Appl. 2020 Jul;112:110922. doi: 10.1016/j.msec.2020.110922. Epub 2020 Apr 4.
Green nanotechnology plays a significant role in developing effective treatment strategies for numerous diseases. The biological synthesis of metal nanoparticles (M-NPs) possesses suitable alternatives than chemical techniques. Using plant extract to synthesis M-NPs is an eco-friendly, non-toxic, and cost-effective that are suitable for biological applications and efforts are directed to explore the efficacy of these materials in cancer management. In this study, gold nanoparticles (Au-NPs) were synthesised by following a one-step green synthesis, a reaction between HAuCl and biological molecules present in Tasmannia lanceolata leaf extract as a sole agent for both reduction and stabilisation. The characterisation techniques confirmed the successful synthesis of Au-NPs. TEM photograph revealed spherical shape nanoparticles with an average size of 7.10 ± 0.66 nm. The in-vitro cytotoxicity of Au-NPs was performed by analysing the percentage inhibition of cell viability using Resazurin assay on human liver cancer (HepG2), melanoma cancer (MM418 C1) and breast cancer (MCF-7) cell lines and compared with Au-NPs synthesised by using Backhousia citriodora leaf extract. The results showed that biosynthesised Au-NPs displayed greater inhibitory activity towards MCF-7 cancer cells proliferation compared to HepG2 and MM418 cancer cells. In addition, synthesised Au-NPs@ Tasmannia lanceolata leaf extract indicated higher inhibitory activity towards cancer cells compared to Au-NPs@ Backhousia citriodora leaf extract.
绿色纳米技术在开发针对多种疾病的有效治疗策略方面发挥着重要作用。与化学技术相比，金属纳米粒子（M-NPs）的生物合成具有合适的替代品。使用植物提取物合成 M-NPs 是一种环保、无毒且具有成本效益的方法，适用于生物应用，并且人们正在努力探索这些材料在癌症管理中的功效。在这项研究中，通过一步绿色合成法，即 HAuCl 与 Tasmannia lanceolata 叶提取物中存在的生物分子之间的反应，合成了金纳米粒子（Au-NPs），作为还原和稳定的唯一试剂。特性技术证实了 Au-NPs 的成功合成。TEM 照片显示出具有 7.10 ± 0.66nm 平均粒径的球形纳米粒子。通过使用 Resazurin 测定法分析人肝癌（HepG2）、黑色素瘤（MM418 C1）和乳腺癌（MCF-7）细胞系中细胞活力抑制百分比来评估 Au-NPs 的体外细胞毒性，并与使用 Backhousia citriodora 叶提取物合成的 Au-NPs 进行比较。结果表明，与 HepG2 和 MM418 癌细胞相比，生物合成的 Au-NPs 对 MCF-7 癌细胞增殖具有更大的抑制活性。此外，与来自 Backhousia citriodora 叶提取物的 Au-NPs 相比，来自 Tasmannia lanceolata 叶提取物的 Au-NPs@ Tasmannia lanceolata 叶提取物对癌细胞表现出更高的抑制活性。
