利用美人蕉花胶合成银纳米粒子和金纳米粒子及其对细菌的药物活性和抗癌功效。

Biosynthesis of silver and gold nanoparticles using Musa acuminata colla flower and its pharmaceutical activity against bacteria and anticancer efficacy.

机构信息

Department of Plant Biology & Biotechnology, Loyola College (Affiliated to Madras University), Chennai 600 034, Tamil Nadu, India.

出版信息

J Photochem Photobiol B. 2019 Dec;201:111670. doi: 10.1016/j.jphotobiol.2019.111670. Epub 2019 Oct 31.

DOI:10.1016/j.jphotobiol.2019.111670

PMID:31706087

Abstract

Synthesis of nanoparticles using plant sources as reducing agent has become important, as physical and chemical methods are costlier and affects environment. Hence it is important to develop environment friendly nanoparticle synthesis by avoiding the use of toxic chemicals. The present study aimed to synthesize silver nanoparticles (Ag Nps) and gold nanoparticles (AuNps) using Musa acuminata colla flower and its pharmaceutical activity against extended spectrum beta-lactamase (ESBL) gene producing bacteria and anticancer efficacy. The synthesized Ag and Au NPs were analysed by means of UV-Vis, FTIR, XRD,SEM and EDAX evidenced the bioreduction of Ag ions to Ag and Au ions to Au respectively. Both nanoparticles and flower extracts were studied for antibacterial activity of ESBL gene producing bacteria by disc diffusion and microdilution (Resazurin) method. In vitro anticancer efficacy (MCF-7) and toxicity (VERO) of AgNPs, AuNPs, aqueous extract and ethanol extract of flowers were performed by MTT assay. IC50 value for DPPH analysis was at 390 μg and 460 μg for ethanol and aqueous extract respectively. Total antioxidant content was found be 740 μg/mg and 460 μg/mg for ethanol and aqueous extract. GCMS analysis authenticated the existence of the compounds namely, 9,12-octadecadienoic acid(z,z)- and n-hexadecanoic acid in the crude extract of the samples. Among the samples, AgNPs had best antibacterial activity. AgNPs and AuNPs were confirmed by colour change to reddish brown and ruby red. Further ƛmax were obtained at 474 and 540 nm by UV - visible spectrum. SEM analysis revealed the particle size ranges from 12.6 to 15.7 nm for silver and 10.1 to 15.6 nm for gold nanoparticles. The EDAX spectrum shows a strong signal for elemental Ag and Au at ~ 3 keV and 1.5 keV. The XRD patterns for silver and gold nanoparticles at 36.701, 42.900, 63.281 and 76.398 corresponding to the lattice planes 2.4467, 2.1064, 1.46839, 1.24564 nm and 27.32, 36.7228, 39.56, 42.888, 63.253, 63.253, 65.02 and 76.383 corresponding to the lattice planes 3.262, 2.44530, 2.276, 2.1070, 1.46897, 1.4332 and 1.24585 nm. The IC50 values for MCF-7 and VERO cells were 30.0 μg/ml and 55.0 μg/ml respectively.

摘要

使用植物来源作为还原剂合成纳米粒子已变得重要，因为物理和化学方法成本更高，并且会影响环境。因此，通过避免使用有毒化学物质来开发环保型纳米粒子合成方法非常重要。本研究旨在使用 Musa acuminata colla 花合成银纳米粒子（AgNps）和金纳米粒子（AuNps），并研究其对产超广谱β-内酰胺酶（ESBL）基因的细菌的药物活性和抗癌功效。通过紫外可见光谱、傅里叶变换红外光谱、X 射线衍射、扫描电子显微镜和能谱分析（EDAX）证实了 Ag 离子还原为 Ag，Au 离子还原为 Au 的生物还原。通过圆盘扩散和微量稀释（Resazurin）法研究了合成的 Ag 和 Au 纳米粒子以及花提取物对产 ESBL 基因的细菌的抗菌活性。通过 MTT 测定法测定了 AgNPs、AuNPs、花的水提物和醇提物对 MCF-7（人乳腺癌细胞）和 VERO（非洲绿猴肾细胞）的体外抗癌活性和毒性。DPPH 分析的 IC50 值分别为 390μg 和 460μg 的乙醇和水提取物。总抗氧化含量分别为 740μg/mg 和 460μg/mg 的乙醇和水提取物。GCMS 分析证实了存在化合物，即 9,12-十八碳二烯酸（z,z）-和正十六烷酸在样品的粗提物中。在样品中，AgNPs 具有最佳的抗菌活性。AgNPs 和 AuNPs 通过颜色变为红棕色和红宝石红色来确认。进一步的ƛmax 通过紫外-可见光谱在 474 和 540nm 处获得。SEM 分析表明，银纳米粒子的粒径范围为 12.6 至 15.7nm，金纳米粒子的粒径范围为 10.1 至 15.6nm。EDAX 光谱显示在约 3keV 和 1.5keV 处的 Ag 和 Au 的强信号。银和金纳米粒子的 XRD 图谱在 36.701、42.900、63.281 和 76.398 处分别对应于晶格平面 2.4467、2.1064、1.46839、1.24564nm 和 27.32、36.7228、39.56、42.888、63.253、63.253、65.02 和 76.383 对应于晶格平面 3.262、2.44530、2.276、2.1070、1.46897、1.4332 和 1.24585nm。MCF-7 和 VERO 细胞的 IC50 值分别为 30.0μg/ml 和 55.0μg/ml。