INRAE, University of Bordeaux, UMR BFP, 33140, Villenave d'Ornon, France.
Department of Cell Biology and Genetics, Faculty of Science, Palacký University in Olomouc, Šlechtitelů 27, 783 71, Olomouc-Holice, Czech Republic.
Arch Virol. 2020 Jul;165(7):1711-1714. doi: 10.1007/s00705-020-04656-w. Epub 2020 May 14.
Double-stranded RNA and total RNA purified from sour cherry leaves (Prunus cerasus, cv. Amarelka Chvalkovicka) was analyzed by high-throughput sequencing. BLAST annotation identified contigs with homology to several already known cherry-infecting viruses (prune dwarf virus, prunus necrotic ringspot virus, prunus virus F, little cherry virus 1) as well as contigs with sequences more distantly related to those of members of the family Betaflexiviridae and in particular to prunus virus T of the genus Tepovirus. The full genome sequence of a putative virus (6,847 nucleotides [nt]; GenBank no. MT090966) was assembled and completed at the genome ends. The genome has a typical tepovirus organization, containing three overlapping open reading frames (ORFs), encoding a replication-associated protein, a movement protein and a capsid protein, respectively. Both its genome organization and its phylogenetic relationships show that the virus belongs to the genus Tepovirus, but considering the species demarcation criteria for the family Betaflexiviridae, it appears to represent a novel virus species, and we propose the name "cherry virus T" (ChVT) for this virus.
从酸樱桃叶片（Prunus cerasus，cv. Amarelka Chvalkovicka）中提取的双链 RNA 和总 RNA 通过高通量测序进行了分析。BLAST 注释鉴定出与几种已发现的樱桃感染病毒（李矮缩病毒、李坏死环斑病毒、李病毒 F、樱桃李病毒 1）同源的序列，以及与贝塔 Flexiviridae 家族成员以及特别是李病毒 T 属（Tepovirus）成员的序列关系较远的序列。组装并完成了一个假定病毒（6847 个核苷酸[nt]；GenBank 编号 MT090966）的全长基因组序列。该基因组具有典型的 Tepovirus 组织，包含三个重叠的开放阅读框（ORF），分别编码复制相关蛋白、运动蛋白和衣壳蛋白。该病毒的基因组组织及其系统发育关系表明，它属于 Tepovirus 属，但考虑到贝塔 Flexiviridae 科的种划分标准，它似乎代表了一种新的病毒种，我们提议将该病毒命名为“樱桃病毒 T”（ChVT）。
