Xylogianni Evanthia, Margaria Paolo, Knierim Dennis, Sareli Kyriaki, Winter Stephan, Chatzivassiliou Elisavet K
Plant Pathology Laboratory, Department of Crop Science, School of Agricultural Production, Infrastructure and Environment, Agricultural University of Athens, Iera Odos 75, Votanikos, 11855 Athens, Greece.
Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, 38124 Braunschweig, Germany.
Pathogens. 2021 May 8;10(5):574. doi: 10.3390/pathogens10050574.
Field surveys were conducted in Greek olive orchards from 2017 to 2020 to collect information on the sanitary status of the trees. Using a high-throughput sequencing approach, viral sequences were identified in total RNA extracts from several trees and assembled to reconstruct the complete genomes of two isolates of a new viral species of the genus (), for which the name olive virus T (OlVT) is proposed. A reverse transcription-polymerase chain reaction assay was developed which detected OlVT in samples collected in olive growing regions in Central and Northern Greece, showing a virus prevalence of 4.4% in the olive trees screened. Sequences of amplified fragments from the movement-coat protein region of OlVT isolates varied from 75.64% to 99.35%. Three olive varieties (Koroneiki, Arbequina and Frantoio) were infected with OlVT via grafting to confirm a graft-transmissible agent, but virus infections remained latent. In addition, cucumber mosaic virus, olive leaf yellowing-associated virus and cherry leaf roll virus were identified.
2017年至2020年期间，在希腊的橄榄园中进行了实地调查，以收集有关树木卫生状况的信息。采用高通量测序方法，在几棵树的总RNA提取物中鉴定出病毒序列，并进行组装以重建一个新病毒种（）两个分离株的完整基因组，为此提议将其命名为橄榄病毒T（OlVT）。开发了一种逆转录-聚合酶链反应检测方法，该方法在希腊中部和北部橄榄种植区采集的样本中检测到了OlVT，在所筛查的橄榄树中病毒患病率为4.4%。OlVT分离株移动蛋白-外壳蛋白区域扩增片段的序列相似度在75.64%至99.35%之间。通过嫁接将三种橄榄品种（科罗内基、阿贝基纳和佛朗托伊奥）感染OlVT以确认一种可通过嫁接传播的病原体，但病毒感染仍处于潜伏状态。此外，还鉴定出了黄瓜花叶病毒、橄榄叶黄化相关病毒和樱桃叶卷病毒。
