“VA”，一种新型红细胞多凝集现象，其特征为H受体受抑并与溶血性贫血相关。II. 免疫荧光、电子显微镜、细胞电泳及生物化学观察

'VA', a new type of erythrocyte polyagglutination characterized by depressed H receptors and associated with hemolytic anemia. II. Observations by immunofluorescence, electron microscopy, cell electrophoresis and Biochemistry.

作者信息

Graninger W, Poschmann A, Fischer K, Schedl-Giovannoni I, Hörandner H, Klaushofer K

出版信息

Vox Sang. 1977;32(4):201-7. doi: 10.1111/j.1423-0410.1977.tb00630.x.

DOI:10.1111/j.1423-0410.1977.tb00630.x

Abstract

With help of immunoflorescence, best with anti-AHP from Helix pomatia, a stippled structure could be demonstrated on the patient"s red blood cells. Thus an "A-like" receptor could be detected on the erythrocyte membrane of this group O patient. The reactive antigen was proved not to be a crypt antigen exposed by the action of neuraminidase. The same stippled fluorescence with antiAhp was observed on the red blood cells of a patient suffering from hemolytic anemia induced by influnza A2 virus. In this case this virus was shown not to be responsible for polyagglutination. No virus or microorganism could be isolated from the patient"s blood. Also by immunofluorescence the weak expression of the H antigen could be demonstrated with an extract of Evonymus europaeus. Electron microscopy of erythrocytes was normal. The neuraminic acid content and the electrophoretic mobility were found to be decreased to a minor degree. No distinct cell populations could be observed.

摘要

借助免疫荧光技术，最好使用来自苹果扁卷螺的抗AHP，在患者的红细胞上可显示出一种斑点状结构。因此，在这名O型血患者的红细胞膜上可检测到一种“类A”受体。已证实反应性抗原不是由神经氨酸酶作用而暴露的隐蔽抗原。在一名由A2型流感病毒引起的溶血性贫血患者的红细胞上，观察到了与抗Ahp相同的斑点状荧光。在这种情况下，该病毒被证明与多凝集无关。未从患者血液中分离出病毒或微生物。同样通过免疫荧光，用欧洲卫矛提取物可证明H抗原的弱表达。红细胞的电子显微镜检查正常。发现神经氨酸含量和电泳迁移率有轻微下降。未观察到明显的细胞群体。