Halbhuber K J, Geyer G, Feuerstein H
Folia Haematol Int Mag Klin Morphol Blutforsch. 1977;104(1):85-97.
The present findings proved Alcian Blue as hemagglutinin suitable for the specific demonstration of negatively charged sites of the cell surface. Erythrocytes with reduced surface charge because of previous trypsination or desialization exhibited decreased Alcian Blue agglutination. On the other hand, Alcian Blue agglutinates more intensely red blood cells with a high surface charge (reticulocytes). Findings with A antiserum or the lectins prepared from Lens culinaris and Helix pomatia indicate a masking of glycolipidic group A receptors by glycoproteins of the erythrocyte membrane. Effects of enzymatic degradation (neuraminidase, trpysin, pronase) combined with incubation in NaF-medium for the deprivation of ATP could be explained by a rearrangement of receptors resulting in altered agglutinability of erythrocytes.
目前的研究结果证明阿尔辛蓝作为血凝素适用于特异性显示细胞表面带负电荷的位点。由于先前的胰蛋白酶处理或去唾液酸化而导致表面电荷减少的红细胞表现出阿尔辛蓝凝集作用降低。另一方面，阿尔辛蓝对表面电荷高的红细胞（网织红细胞）凝集作用更强。用抗A血清或由菜豆和苹果螺制备的凝集素进行的研究结果表明红细胞膜糖蛋白对糖脂A受体具有掩盖作用。酶促降解（神经氨酸酶、胰蛋白酶、链霉蛋白酶）与在氟化钠培养基中孵育以剥夺ATP的作用可通过受体重排导致红细胞凝集性改变来解释。
