印度尼西亚一种不典型眼部弓形虫病中出现的弓形体 SAG2 型 III。
Toxoplasma gondii SAG2 type III in an atypical presentation of ocular toxoplasmosis in Indonesia.
机构信息
Department of Parasitology Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
Department of Parasitology Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
出版信息
Int J Infect Dis. 2020 Jul;96:440-444. doi: 10.1016/j.ijid.2020.05.017. Epub 2020 May 12.
OBJECTIVE
This study aimed to perform genotyping of Toxoplasma gondii strain or variant causing atypical toxoplasmic uveitis in Indonesian patients.
METHODS
Ocular fluid samples originating from 46 uveitis patients with non-specific ocular manifestations were analysed for Toxoplasma infection by PCR of the B1 locus. The clonal type was determined by amplification, sequencing and phylogenetic analysis of SAG2 and GRA6 loci in B1-positive samples. Clinical data were obtained from the medical records.
RESULTS
Pan uveitis was the most frequent manifestation (65.2%) and mostly unilateral (76.1%). PCR of the B1 locus identified eight positive subjects (12.5%); six had panuveitis and two of these had diabetes mellitus. Phylogenetic analysis with maximum likelihood of the SAG2 locus in the B1-positive samples resulted in Toxoplasma gondii SAG2 type III allele. No positive result was obtained from the PCR of GRA6 locus.
CONCLUSION
Toxoplasma gondii SAG2-type III allele was identified in an atypical presentation of toxoplasmic uveitis in Indonesia.
目的
本研究旨在对引起印度尼西亚患者非典型弓形虫性葡萄膜炎的弓形虫株或变体进行基因分型。
方法
对 46 例具有非特异性眼部表现的葡萄膜炎患者的眼内液样本进行 PCR 检测 B1 基因座以分析弓形虫感染情况。在 B1 阳性样本中扩增、测序和系统进化分析 SAG2 和 GRA6 基因座，以确定克隆类型。临床数据来自病历。
结果
全葡萄膜炎是最常见的表现（65.2%），且多为单侧（76.1%）。B1 基因座 PCR 鉴定出 8 名阳性患者（12.5%）；其中 6 名患有全葡萄膜炎，其中 2 名患有糖尿病。对 B1 阳性样本进行 SAG2 基因座最大似然法的系统进化分析，结果为弓形虫 SAG2 型 III 等位基因。GRA6 基因座 PCR 未获得阳性结果。
结论
在印度尼西亚，非典型弓形虫性葡萄膜炎的表现中鉴定出了弓形虫 SAG2 型 III 等位基因。
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