对 20 例眼弓形体病患者眼液样本的弓形虫直接基因分型：法国以 II 型为主。
Direct genotyping of Toxoplasma gondii in ocular fluid samples from 20 patients with ocular toxoplasmosis: predominance of type II in France.
机构信息
AP-HP, Groupe Hospitalier Pitié-Salpêtrière, Service Parasitologie-Mycologie, Paris F-75013 France.
出版信息
J Clin Microbiol. 2011 Apr;49(4):1513-7. doi: 10.1128/JCM.02196-10. Epub 2011 Jan 19.
Abstract
We report the direct genotyping analysis of Toxoplasma gondii in ocular samples collected from 20 patients, as well as associated clinical and epidemiological data. This work was aimed at better understanding the impact of genotypes of Toxoplasma gondii strains on toxoplasmic retinochoroiditis. For this purpose, we studied the aqueous humor (AH) or vitreous humor (VH) of 20 patients presenting with ocular toxoplasmosis (OT) in 2 hospitals in France. Genetic characterization was obtained with microsatellite markers in a multiplex PCR assay. In contrast to the results of previous studies, we found no association between atypical Toxoplasma gondii genotypes and the occurrence of OT. Considering the local epidemiological data, our OT patients seemed to be infected more frequently by ordinary type II strains found in the environment. In conclusion, direct genotyping of Toxoplasma gondii strains from aqueous or vitreous humor showed a predominance of the type II genotype in ocular toxoplasmosis; this may be due to a high exposure rate of this genotype in humans.
摘要
我们报告了 20 名患者眼部样本中刚地弓形虫的直接基因分型分析，以及相关的临床和流行病学数据。这项工作旨在更好地了解刚地弓形虫株基因型对弓形虫性视网膜炎的影响。为此，我们研究了法国 2 家医院 20 名眼部弓形虫病（OT）患者的房水（AH）或玻璃体（VH）。采用微卫星标记物的多重 PCR 检测法进行基因特征分析。与之前研究的结果相反，我们没有发现非典型刚地弓形虫基因型与 OT 发生之间存在关联。考虑到当地的流行病学数据，我们的 OT 患者似乎更频繁地被环境中发现的普通 II 型菌株感染。总之，从房水或玻璃体中直接对刚地弓形虫株进行基因分型显示，OT 中 II 型基因型占优势；这可能是由于该基因型在人类中的高暴露率所致。
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