Department of Surgery, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo, Brazil.
Department of Surgery, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo, Brazil.
Res Vet Sci. 2020 Aug;131:222-231. doi: 10.1016/j.rvsc.2020.05.005. Epub 2020 May 6.
Skin lesions are normal to all species, regardless of gender or age. The skin, the largest organ of the body, has function as a primary barrier to the chemical, physical and biological aggressions of the environment. In animals, these lesions may be due to fights and/or predations, also as in humans, there is a very common cause of dermal lesions that are caused by burns and carcinomas. Looking for new techniques of tissue bioengineering, studies have been shown promising results for formulations of acellular biological scaffolds from tissue decellularization for the reconstitution of these lesions. The decellularization has its proof by a varied range of tests such as scanning electron microscopy and residual genomic DNA tests. Subsequently the tissue can go through the process of recellularization using cells of interest, even the animal that will receive this tissue, reducing the risks of rejection and improving the response to tissue transplantation. Thus, this manuscript aimed at the decellularization of the tissue with the use of chemical and physical means followed by sterilization and the establishment of a protocol for the recellularization of a decellularized scaffold from the Wistar rat dermis using murine fibroblasts and mesenchymal stem cells from canine adipose tissue for 7 days. After efficacy tests, the tissue recellularization were confirmed by immunofluorescence assays and scanning electron microscopy where the adherence of the cells in the biological scaffold was observed.
皮肤损伤在所有物种中都很常见，与性别或年龄无关。皮肤作为人体最大的器官，具有抵御环境中化学、物理和生物侵袭的主要屏障功能。在动物中，这些损伤可能是由于打架和/或捕食，就像人类一样，有一种非常常见的皮肤损伤原因是烧伤和癌。为了寻找组织生物工程的新技术，研究表明，从组织去细胞化制备无细胞生物支架的配方对于这些损伤的重建具有有前景的结果。去细胞化通过各种测试得到证明，如扫描电子显微镜和残留基因组 DNA 测试。随后，组织可以通过使用感兴趣的细胞进行再细胞化，甚至可以使用将接受这种组织的动物的细胞进行再细胞化，从而降低排斥反应的风险，提高组织移植的反应。因此，本文旨在使用化学和物理方法对组织进行去细胞化，然后进行消毒，并建立使用来自犬脂肪组织的鼠成纤维细胞和间充质干细胞对 Wistar 大鼠真皮去细胞化支架进行 7 天再细胞化的方案。在进行功效测试后，通过免疫荧光分析和扫描电子显微镜观察到细胞在生物支架中的黏附来确认组织再细胞化。
